Natural Products & Nanobiotechnology Research Lab, Department of Community Medicine, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), (Saveetha Deemed to be University), Thandalam, Chennai, 600 105, Tamil Nadu, India.
Centre for Advanced Research, Institute of Biotechnology, Saveetha School of Engineering (SSE), Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Thandalam, Chennai, 602105, Tamil Nadu, India.
Microb Pathog. 2024 May;190:106635. doi: 10.1016/j.micpath.2024.106635. Epub 2024 Apr 3.
The plant Erythrina indica comes under Fabaceae family, mainly used for used in traditional medicine as nervine sedative, antiepileptic, antiasthmatic, collyrium in opthalmia, antiseptic. Current study focused synthesize of silver nanoparticles (AgNPs) by E. indica leaf ethanol extract. The green-synthesized AgNPs underwent characterization using multiple analytical techniques, including UV-visible, FTIR, DLS, SEM, TEM, XRD, and EDX, and estimation of their antioxidant activity and antimicrobial activity. Phytochemical analysis identified alkaloids, tannins, saponins, flavonoids, and phenols as secondary metabolites. The Total Phenol Content (TPC) was determined to be 237.35 ± 2.02 mg GAE-1, indicating a substantial presence of phenolic compounds. The presence of AgNPs was verified through UV-Visible analysis at 420 nm, and FT-IR revealed characteristic phenolic functional groups. DLS analysis indicated a narrow size distribution (polydispersity index - PDI: 3.47%), with SEM revealing spherical AgNPs of approximately 20 nm. TEM showed homogeneous, highly polycrystalline AgNPs with lattice spacing at 0.297. XRD analysis demonstrated crystallinity and purity, with distinct reflection peaks corresponding to miller indices of JCPDS card no. 01 087 1473. In vitro, AgNPs exhibited robust antioxidant activity like; DPPH, ABTS, and HO, surpassing E. indica-assisted synthesis. ABTS assay indicated higher antioxidant activity (81.94 ± 0.05%) for AgNPs at 734 nm, while E. indica extraction showed 39.67 ± 0.07%. At 532 nm, both E. indica extraction (57.71 ± 0.11%) and AgNPs (37.41 ± 0.17%) exhibited HO scavenging. Furthermore, AgNPs displayed significant antimicrobial properties, inhibiting Staphylococcus aureus (15.7 ± 0.12 mm) and Candida albicans (10.7 ± 0.17 mm) byfor the concentration of 80 μg/mL. Through the characterizations underscore of the potential of Erythrina indica-synthesized AgNPs, rich in polyphenolic compounds, for pharmacological, medical, biological applications and antipyretic properties.
苏木属于豆科植物，主要用于传统医学，具有神经镇静、抗癫痫、抗哮喘、眼结膜炎、防腐剂等作用。本研究主要集中在苏木叶乙醇提取物合成银纳米粒子（AgNPs）。通过多种分析技术，包括紫外-可见光谱、傅里叶变换红外光谱、动态光散射、扫描电子显微镜、透射电子显微镜、X 射线衍射和能谱分析，对绿色合成的 AgNPs 进行了表征，并对其抗氧化活性和抗菌活性进行了评估。植物化学分析鉴定出生物碱、单宁、皂苷、类黄酮和酚类化合物为次生代谢物。总酚含量（TPC）测定为 237.35±2.02mgGAE-1，表明酚类化合物含量较高。通过 420nm 的紫外-可见分析验证了 AgNPs 的存在，傅里叶变换红外光谱显示了特征酚类官能团。动态光散射分析表明粒径分布较窄（多分散指数-PDI：3.47%），扫描电子显微镜显示约 20nm 的球形 AgNPs。透射电子显微镜显示均匀、高度多晶的 AgNPs，晶格间距为 0.297。X 射线衍射分析表明结晶度和纯度高，具有对应于 JCPDS 卡号 010871473 的米勒指数的明显反射峰。体外，AgNPs 表现出很强的抗氧化活性，如 DPPH、ABTS 和 HO，超过了苏木辅助合成的活性。ABTS 法测定表明，在 734nm 时，AgNPs 的抗氧化活性更高（81.94±0.05%），而苏木提取液的抗氧化活性为 39.67±0.07%。在 532nm 时，苏木提取液（57.71±0.11%）和 AgNPs（37.41±0.17%）均表现出对 HO 的清除作用。此外，AgNPs 表现出显著的抗菌特性，在 80μg/mL 浓度下抑制金黄色葡萄球菌（15.7±0.12mm）和白色念珠菌（10.7±0.17mm）。通过苏木合成的富含多酚类化合物的 AgNPs 的特性强调，它们具有药理学、医学、生物学应用和退热特性。
