Translational Research Institute, AdventHealth, Orlando, Florida, United States.
Division of Diabetes Endocrinology and Metabolism, Johns Hopkins University School of Medicine, Institute for Fundamental Biomedical Research, Johns Hopkins All Children's Hospital, St. Petersburg, Florida, United States.
Am J Physiol Cell Physiol. 2024 Apr 1;326(4):C1248-C1261. doi: 10.1152/ajpcell.00726.2023. Epub 2024 Mar 4.
Adipose-derived stem cells (ADSCs) play an important role in the differential capacity for excess energy storage between upper body abdominal (ABD) adipose tissue (AT) and lower body gluteofemoral (GF) AT. We cultured ADSCs from subcutaneous ABD AT and GF AT isolated from eight women with differential body fat distribution and performed single-cell RNA sequencing. Six populations of ADSCs were identified and segregated according to their anatomical origin. The three ADSC subpopulations in GF AT were characterized by strong cholesterol/fatty acid (FA) storage and proliferation signatures. The two ABD subpopulations, differentiated by higher expression of committed preadipocyte marker genes, were set apart by differential expression of extracellular matrix and ribosomal genes. The last population, identified in both depots, was similar to smooth muscle cells and when individually isolated and cultured in vitro they differentiated less than the other subpopulations. This work provides important insight into the use of ADSC as an in vitro model of adipogenesis and suggests that specific subpopulations of GF-ADSCs contribute to the more robust capacity for GF-AT to expand and grow compared with ABD-AT in women. Identification of distinct subpopulations of adipose-derived stem cells (ADSCs) in upper body abdominal subcutaneous (ABD) and lower body gluteofemoral subcutaneous (GF) adipose tissue depots. In ABD-ADSCs, subpopulations are more committed to adipocyte lineage. GF-ADSC subpopulations are enriched for genes involved in lipids and cholesterol metabolism. Similar depot differences were found in stem cell population identified in freshly isolated stoma vascular fraction. The repertoire of ADSCs subpopulations was different in apple-shaped versus pear-shaped women.
脂肪来源干细胞（ADSCs）在人体上半身腹部（ABD）脂肪组织（AT）和下半身臀股部（GF）AT 之间储存多余能量的能力差异方面发挥着重要作用。我们从 8 位具有不同体脂分布的女性身上分离的 ABD AT 和 GF AT 中培养 ADSC，并进行了单细胞 RNA 测序。根据其解剖起源，我们鉴定并分离了 6 种 ADSC 群体。GF AT 中的三个 ADSC 亚群具有较强的胆固醇/脂肪酸（FA）储存和增殖特征。两个 ABD 亚群分化程度更高，其特征是表达预先确定的脂肪细胞标记基因，它们通过细胞外基质和核糖体基因的差异表达而分离。最后一个群体在两个脂肪组织中都存在，类似于平滑肌细胞，当它们在体外单独分离和培养时，分化程度低于其他亚群。这项工作为 ADSC 作为体外脂肪生成模型的应用提供了重要的见解，并表明 GF-ADSC 的特定亚群有助于 GF-AT 与 ABD-AT 相比具有更强大的扩张和生长能力。在上半身腹部皮下（ABD）和下半身臀股部皮下（GF）脂肪组织中鉴定出不同的脂肪来源干细胞（ADSCs）亚群。在 ABD-ADSCs 中，亚群更倾向于向脂肪细胞谱系分化。GF-ADSC 亚群富含参与脂质和胆固醇代谢的基因。在新分离的腔隙血管部分中也发现了与干细胞群体有关的类似脂肪组织差异。苹果形和梨形女性的 ADSC 亚群组成不同。
