miR-590-3p/DKK1 轴对肾母细胞瘤进展的影响。

Effect of miR-590-3p/DKK1 Axis on the Progression of Wilms' Tumour.

机构信息

Department of Clinical Laboratory, Yantaishan Hospital, 10087 Yantai, Shandong, China.

出版信息

Arch Esp Urol. 2024 Mar;77(2):135-141. doi: 10.56434/j.arch.esp.urol.20247702.18.

DOI:10.56434/j.arch.esp.urol.20247702.18

PMID:38583005

Abstract

BACKGROUND

Wilms' tumour is the most prevalent abdominal malignancy in children. This study focused on the mechanism of the /Dickkopf 1 () axis in Wilms' tumour.

METHODS

The mRNA levels of and in 49 pairs of Wilms' tumour pathological specimens and normal tissues were determined using real-time quantitative polymerase chain reaction (RT-qPCR). Wilms' tumour cells' invasion ability and proliferative ability were assessed using a Transwell assay and Cell Counting Kit-8 (CCK-8) assay, respectively. Dual-luciferase assay was performed to evaluate the potential relationship between and in Wilms tumour. Furthermore, a mouse transplanted tumour model was constructed to explore the function of inhibitor on Wilms' tumour growth .

RESULTS

emerged as a target gene of in Wilms' tumour. expression was downregulated ( < 0.01), but was overexpressed ( < 0.01) in Wilms' tumour tissues compared to normal tissues. overexpression accelerated Wilms' tumour invasive ability and cell proliferation ( < 0.01). Additionally, partially reversed -induced proliferation ( < 0.05) and invasion ability ( < 0.01). Furthermore, downregulation of restrained the growth rate of transplanted tumours in nude mice ( < 0.01).

CONCLUSIONS

Through the regulation of , accelerated the invasion and proliferation of Wilms' tumour. The study suggests that the / axis represents a novel mechanism in Wilms' tumour.

摘要

背景

肾母细胞瘤是儿童最常见的腹部恶性肿瘤。本研究聚焦于 Dickkopf 1 () 轴在肾母细胞瘤中的作用机制。

方法

采用实时定量聚合酶链反应（RT-qPCR）检测 49 对肾母细胞瘤病理标本和正常组织中和的 mRNA 水平。通过 Transwell 实验和细胞计数试剂盒-8（CCK-8）实验分别评估肾母细胞瘤细胞的侵袭能力和增殖能力。双荧光素酶报告基因实验评估与在肾母细胞瘤中的潜在关系。此外，构建了小鼠移植瘤模型，以探讨 Dickkopf 1 抑制剂对肾母细胞瘤生长的作用。

结果

结果表明，在肾母细胞瘤中，是的靶基因。与正常组织相比，肾母细胞瘤组织中表达下调（ < 0.01），而过表达（ < 0.01）。过表达可加速肾母细胞瘤的侵袭能力和细胞增殖（ < 0.01）。此外，部分逆转了 -诱导的增殖（ < 0.05）和侵袭能力（ < 0.01）。此外，下调可抑制裸鼠移植瘤的生长速度（ < 0.01）。