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长链非编码RNA TPRG1-AS1在宫颈鳞状细胞癌发生发展中的作用及其预后价值

Role of lncRNA TPRG1-AS1 in the development of cervical squamous cell carcinoma and its prognostic value.

作者信息

Fan Yang, Ye Lan, Wang Shuyu, Wang Junwei, Wang Ke, Li Ying

机构信息

People's Hospital of Ningxia Hui Autonomous Region, Ningxia Medical University, Ningxia, 750011, China.

Department of Gynecology, Shanghai Putuo Maternity & Infant Health Hospital, Shanghai, 200062, China.

出版信息

Discov Oncol. 2024 Dec 18;15(1):754. doi: 10.1007/s12672-024-01654-1.

DOI:10.1007/s12672-024-01654-1
PMID:39692778
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11655740/
Abstract

OBJECTIVE

Cervical squamous cell carcinoma (CSCC) has a poor prognosis due to persistent HPV infection. LncRNA TPRG1-AS1 is linked to regulating the development of many cancers, so the regulatory mechanism and prognostic value of TPRG1-AS1 in CSCC were explored.

METHODS

138 patients with cervical cancer were included. TPRG1-AS1 expression and miR-590-3p were analyzed by qRT-PCR. The association between TPRG1-AS1 and clinicopathological features was investigated. Independent prognostic factors of CSCC were analyzed by multifactorial Cox regression. Patient survival was analyzed using Kaplan-Meier plotter curves. CCK-8 was employed to evaluate the proliferative capacity of the cells. Transwell assays were performed to evaluate the effects of TPRG1-AS1 and miR-590-3p on cell migration and invasion performance, and the target of both was reported by DLR assay.

RESULTS

TPRG1-AS1 levels were ascended in CSCC, and miR-590-3p levels were reduced. TPRG1-AS1 and miR-590-3p target binding and expression correlated negatively. Knockdown of TPRG1-AS1 expression could facilitate high miR-590-3p expression, which reduced cell proliferation, migration, and invasion ability. TPRG1-AS1 is an independent prognostic factor.

CONCLUSION

TPRG1-AS1 has potential as a prognostic marker for CSCC. Silencing the expression of TPRG1-AS1 could contribute to the high miR-590-3p expression thereby slowing down the progression of CSCC.

摘要

目的

由于持续性人乳头瘤病毒（HPV）感染，宫颈鳞状细胞癌（CSCC）预后较差。长链非编码RNA（lncRNA）TPRG1-AS1与多种癌症的发生发展调控相关，因此本研究探讨TPRG1-AS1在CSCC中的调控机制及预后价值。

方法

纳入138例宫颈癌患者。采用qRT-PCR分析TPRG1-AS1表达及miR-590-3p水平。研究TPRG1-AS1与临床病理特征之间的关联。通过多因素Cox回归分析CSCC的独立预后因素。采用Kaplan-Meier生存曲线分析患者生存率。使用CCK-8评估细胞增殖能力。进行Transwell实验以评估TPRG1-AS1和miR-590-3p对细胞迁移和侵袭能力的影响，并通过双荧光素酶报告基因实验验证二者的靶向关系。

结果

CSCC中TPRG1-AS1水平升高，miR-590-3p水平降低。TPRG1-AS1与miR-590-3p靶向结合且表达呈负相关。敲低TPRG1-AS1表达可促进miR-590-3p高表达，进而降低细胞增殖、迁移和侵袭能力。TPRG1-AS1是独立预后因素。

结论

TPRG1-AS1有望成为CSCC的预后标志物。沉默TPRG1-AS1表达可促进miR-590-3p高表达，从而减缓CSCC进展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9d2/11655740/50bbd219c787/12672_2024_1654_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9d2/11655740/8942583aa540/12672_2024_1654_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9d2/11655740/52c32c187583/12672_2024_1654_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9d2/11655740/fbfdb2a64640/12672_2024_1654_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9d2/11655740/0a35cb085a98/12672_2024_1654_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9d2/11655740/50bbd219c787/12672_2024_1654_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9d2/11655740/8942583aa540/12672_2024_1654_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9d2/11655740/52c32c187583/12672_2024_1654_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9d2/11655740/fbfdb2a64640/12672_2024_1654_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9d2/11655740/0a35cb085a98/12672_2024_1654_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9d2/11655740/50bbd219c787/12672_2024_1654_Fig5_HTML.jpg

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