Song Caixian, Chen Liping
Department of Gynecology and Obstetrics, Wuhan Fourth Hospital, Wuhan 430030, Hubei, China.
Department of Gynecology and Obstetrics, Wuhan Fourth Hospital, No. 76 Jiefang Avenue, Qiaokou District, Wuhan 430030, Hubei, China.
Open Med (Wars). 2024 Mar 13;19(1):20230858. doi: 10.1515/med-2023-0858. eCollection 2024.
Circular RNAs (circRNAs) are key regulators of cervical cancer (CC) progression. This study aimed to elucidate the role and mechanism of circ_0051428, a novel circRNA, in CC tumorigenesis. Quantitative real-time polymerase chain reaction and western blotting analyses confirmed that circ_0051428 and matrix metalloprotein-2 (MMP2) were overexpressed in CC, whereas the microRNA miR-885-3p was poorly expressed. After performing a series of and experiments, circ_0051428 knockdown was shown to repress CC cell invasion and proliferation , and hamper tumor formation . Dual-luciferase reporter and RNA-binding protein immunoprecipitation experiments verified that circ_0051428 interacts with miR-885-3p to regulate the target gene MMP2 of miR-885-3p in CC. In addition, miR-885-3p knockdown offset the anticancer effects of circ_0051428 or MMP2 knockdown on CC cell malignancy. Overall, this study revealed that circ_0051428 executes a tumor-promoting function in CC pathogenesis by modulating the miR-885-3p/MMP2 axis. Our findings provide a novel approach for CC treatment.
环状RNA（circRNAs）是宫颈癌（CC）进展的关键调节因子。本研究旨在阐明一种新型环状RNA circ_0051428在CC肿瘤发生中的作用及机制。定量实时聚合酶链反应和蛋白质免疫印迹分析证实，circ_0051428和基质金属蛋白酶-2（MMP2）在CC中过表达，而微小RNA miR-885-3p表达不足。在进行一系列实验后，circ_0051428敲低显示可抑制CC细胞侵袭和增殖，并阻碍肿瘤形成。双荧光素酶报告基因和RNA结合蛋白免疫沉淀实验证实，circ_0051428与miR-885-3p相互作用，以调节CC中miR-885-3p的靶基因MMP2。此外，miR-885-3p敲低抵消了circ_0051428或MMP2敲低对CC细胞恶性程度的抗癌作用。总体而言，本研究揭示了circ_0051428通过调节miR-885-3p/MMP2轴在CC发病机制中发挥促肿瘤功能。我们的研究结果为CC治疗提供了一种新方法。
