Department of Biosciences, Università degli Studi di Milano, Milan, Italy.
Department of Physics, Politecnico di Milano, Milan, Italy.
Methods Mol Biol. 2024;2798:45-64. doi: 10.1007/978-1-0716-3826-2_4.
In this protocol, we present a noninvasive in planta bioimaging technique for the analysis of hydrogen peroxide (HO) and glutathione redox potential in adult Arabidopsis thaliana plants. The technique is based on the use of stereo fluorescence microscopy to image A. thaliana plants expressing the two genetically encoded fluorescent sensors roGFP2-Orp1 and Grx1-roGFP2. We provide a detailed step-by-step protocol for performing low magnification imaging with mature plants grown in soil or hydroponic systems. This protocol aims to serve the scientific community by providing an accessible approach to noninvasive in planta bioimaging and data analysis.
在本方案中，我们介绍了一种用于分析拟南芥成年植株中过氧化氢（HO）和谷胱甘肽氧化还原电势的非侵入性活体生物成像技术。该技术基于使用立体荧光显微镜对表达两种遗传编码荧光传感器 roGFP2-Orp1 和 Grx1-roGFP2 的拟南芥植株进行成像。我们提供了一个详细的分步方案，用于对在土壤或水培系统中生长的成熟植物进行低倍放大成像。本方案旨在为科学界提供一种易于使用的活体生物成像和数据分析方法。
