Life Science Research Centre, College of Bioresource Sciences, Nihon University, Fujisawa, Japan.
Enzyme Sensor Co. Ltd, Tsukuba, Japan.
Methods Mol Biol. 2024;2798:195-203. doi: 10.1007/978-1-0716-3826-2_13.
Gamma-aminobutyric acid (GABA) is an amino acid that has a role as a signaling molecule. In plants, its involvement in stress responses is widely investigated. A newly developed method of quantification of GABA is described in this chapter. The assay kit consisting of three bacterial enzymes enables easy but accurate measurement of GABA (~200 mg/mL) based on the serial enzymatic reaction leading to dye formation. The method was successfully applied to measure the GABA content in several plant tissues.
γ-氨基丁酸（GABA）是一种氨基酸，作为信号分子发挥作用。在植物中，其在应激反应中的作用得到了广泛的研究。本章介绍了一种新的 GABA 定量方法。该测定试剂盒包含三种细菌酶，通过一系列酶促反应导致染料形成，从而能够轻松、准确地测量 GABA（~200mg/mL）。该方法成功应用于测量几种植物组织中的 GABA 含量。
