用ras或myc癌基因转染的大鼠-1细胞中的糖酵解和甲基氨基异丁酸摄取。
Glycolysis and methylaminoisobutyrate uptake in rat-1 cells transfected with ras or myc oncogenes.
作者信息
Racker E, Resnick R J, Feldman R
出版信息
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3535-8. doi: 10.1073/pnas.82.11.3535.
Abstract
A high rate of aerobic glycolysis was catalyzed by rat-1 cells transfected with a ras oncogene (ras cells); rat-1 cells and rat-1 cells transfected with myc oncogene (myc cells) showed a low rate of glycolysis that was increased after exposure of the cells to type B transforming growth factor (TGF-beta). The uptake of radioactive methylaminoisobutyric acid or L-methionine via system A of amino acid transport also was accelerated after exposure of these cells to TGF-beta, with the myc cells being most sensitive and the ras cells least sensitive. Methionine was found to be a potent inhibitor of glycolysis in ras cells as well as in rat-1 or myc cells that were exposed to TGF-beta. We propose a relationship between the product of the ras oncogene (p21) and the protein(s) induced by exposure to TGF-beta.
摘要
转染了ras癌基因的大鼠-1细胞（ras细胞）催化有氧糖酵解的速率较高；大鼠-1细胞以及转染了myc癌基因的大鼠-1细胞（myc细胞）显示糖酵解速率较低，在细胞暴露于B型转化生长因子（TGF-β）后糖酵解速率增加。这些细胞暴露于TGF-β后，通过氨基酸转运系统A摄取放射性甲基氨基异丁酸或L-甲硫氨酸的过程也加快，其中myc细胞最敏感，ras细胞最不敏感。发现甲硫氨酸是ras细胞以及暴露于TGF-β的大鼠-1或myc细胞中糖酵解的有效抑制剂。我们提出ras癌基因的产物（p21）与暴露于TGF-β诱导的蛋白质之间存在关联。
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