Liu Jiangqi, Xiao Xiaojun, Liao Yun, Xu Xuejie, Liu Yu, Tang Aifa, Zeng Xianhai, Yang Pingchang
Department of Allergy, Longgang ENT Hospital & Shenzhen ENT Institute, Shenzhen, China.
Institute of Allergy & Immunology of Shenzhen University & State Key Laboratory of Respiratory Diseases Allergy Division at Shenzhen University, Shenzhen, China.
Arch Biochem Biophys. 2024 May;755:109984. doi: 10.1016/j.abb.2024.109984. Epub 2024 Apr 6.
Allergen specific immunotherapy (AIT) has been widely used in allergy clinics. The therapeutic effects of it are to be improved. Macrophages occupy the largest proportion of airway immune cells. The aim of this study is to measure the effects of nasal instillation AIT (nAIT) on airway allergy by regulating macrophage functions.
An airway allergy mouse model was established with the ovalbumin-alum protocol. nAIT was conducted for mice with airway allergy through nasal instillation. The effects of nAIT were compared with subcutaneous injection AIT (SCIT) and sublingual AIT (SLIT).
Mice with airway allergy showed the airway allergic response, including lung inflammation, airway hyper responsiveness, serum specific IgE, increase in the amounts of eosinophil peroxidase, mouse mast cell protease-1, and Th2 cytokines in bronchoalveolar lavage fluid. nAIT had a much better therapeutic effect on the airway allergic response than SCIT and SLIT. Mechanistically, we observed better absorption of allergen in macrophages, better production of IL-10 by macrophages, and better immune suppressive functions in macrophages in mice received nAIT than SCIT and SLIT.
The nAIT has a much better therapeutic effect on suppressing the airway allergic response, in which macrophages play a critical role.
变应原特异性免疫疗法（AIT）已在过敏诊所广泛应用，但其治疗效果仍有待提高。巨噬细胞在气道免疫细胞中占比最大。本研究旨在通过调节巨噬细胞功能来评估鼻内滴注AIT（nAIT）对气道过敏的影响。
采用卵清蛋白-明矾方案建立气道过敏小鼠模型。通过鼻内滴注对气道过敏小鼠进行nAIT治疗。将nAIT的效果与皮下注射AIT（SCIT）和舌下AIT（SLIT）进行比较。
气道过敏小鼠表现出气道过敏反应，包括肺部炎症、气道高反应性、血清特异性IgE、支气管肺泡灌洗液中嗜酸性粒细胞过氧化物酶、小鼠肥大细胞蛋白酶-1和Th2细胞因子含量增加。nAIT对气道过敏反应的治疗效果比SCIT和SLIT好得多。从机制上讲，我们观察到接受nAIT的小鼠巨噬细胞中变应原吸收更好，巨噬细胞产生IL-10的能力更强，且巨噬细胞的免疫抑制功能比SCIT和SLIT更好。
nAIT在抑制气道过敏反应方面具有更好的治疗效果，其中巨噬细胞起关键作用。
