Department of Bioinformatics, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal 576104, Karnataka, India.
Institute of Bioinformatics and Applied Biotechnology, Biotech Park, Electronic City, Bengaluru 560100, Karnataka, India.
Genome. 2024 Aug 1;67(8):281-291. doi: 10.1139/gen-2023-0097. Epub 2024 Apr 9.
The human gut is the most favorable niche for microbial populations, and few studies have explored the possibilities of horizontal gene transfer between host and pathogen. Trinucleotide repeat (TNR) expansion in humans can cause more than 40 neurodegenerative diseases. Further, TNRs are a type of microsatellite that resides on coding regions can contribute to the synthesis of homopolymeric amino acids. Hence, the present study aims to estimate the occurrence and diversity of TNRs in bacterial genomes available in the NCBI Genome database. Genome-wide analyses revealed that several bacterial genomes contain different types of uninterrupted TNRs. It was found that TNRs are abundant in the genomes of , and . Interestingly, the genome of strain YBT-1518 contained 169 uninterrupted ATT repeats. The genome of had 46 uninterrupted CAG repeats, which potentially translate into polyglutamine. In some instances, the TNRs were present in genes that potentially encode essential functions. Similar occurrences in human genes are known to cause genetic disorders. Further analysis of the occurrence of TNRs in bacterial genomes is likely to provide a better understanding of mismatch repair, genetic disorders, host-pathogen interaction, and homopolymeric amino acids.
人类肠道是微生物种群最有利的栖息地，很少有研究探索宿主和病原体之间水平基因转移的可能性。三核苷酸重复（TNR）扩张会导致 40 多种神经退行性疾病。此外，TNR 是一种位于编码区域的微卫星，可导致同聚氨基酸的合成。因此，本研究旨在估计 NCBI 基因组数据库中细菌基因组中 TNR 的发生和多样性。全基因组分析显示，几种细菌基因组含有不同类型的不连续 TNR。结果发现，TNR 在 、 和 的基因组中很丰富。有趣的是，菌株 YBT-1518 的基因组中含有 169 个不连续的 ATT 重复序列。的基因组中有 46 个不连续的 CAG 重复序列，可能翻译成多聚谷氨酰胺。在某些情况下，TNR 存在于可能编码必需功能的基因中。已知人类基因中的类似情况会导致遗传疾病。进一步分析细菌基因组中 TNR 的发生情况，可能有助于更好地理解错配修复、遗传疾病、宿主-病原体相互作用和同聚氨基酸。
