State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 66 Changjiang West Road, Qingdao 266580, China.
Shandong Marine Resource and Environment Research Institute, 216 Changjiang Road, Yantai Economic Development Zone, Yantai 264006, China.
Soft Matter. 2024 Apr 24;20(16):3508-3519. doi: 10.1039/d3sm01742g.
The decellularized tilapia skin (dTS) has gained significant attention as a promising material for tissue regeneration due to its ability to provide unique structural and functional components that support cell growth, adhesion, and proliferation. However, the clinical application of dTS is limited by its low mechanical strength and rapid biodegradability. Herein, we prepare a novel RGD (arginine-glycine-aspartic acid) functionalized dTS scaffold (dTS/RGD) by using transglutaminase (TGase) crosslinking. The developed dTS/RGD scaffold possesses excellent properties, including a medium porosity of ∼59.2%, a suitable degradation rate of approximately 80% over a period of two weeks, and appropriate mechanical strength with a maximum tensile stress of ∼46.36 MPa which is much higher than that of dTS (∼32.23 MPa). These properties make the dTS/RGD scaffold ideal for promoting cell adhesion and proliferation, thereby accelerating skin wound healing in a full-thickness skin defect model. Such an enzymatic cross-linking strategy provides a favorable microenvironment for wound healing and holds great potential for application in skin regeneration engineering.
脱细胞罗非鱼皮（dTS）因其能够提供独特的结构和功能成分，支持细胞生长、黏附和增殖，已作为一种很有前途的组织再生材料而受到广泛关注。然而，由于其机械强度低和快速生物降解性，dTS 的临床应用受到限制。在此，我们通过使用转谷氨酰胺酶（TGase）交联作用制备了一种新型的 RGD（精氨酸-甘氨酸-天冬氨酸）功能化 dTS 支架（dTS/RGD）。所开发的 dTS/RGD 支架具有优异的性能，包括约 59.2%的中等孔隙率、约两周内约 80%的适宜降解率以及适当的机械强度，最大拉伸应力约为 46.36 MPa，远高于 dTS（约 32.23 MPa）。这些特性使 dTS/RGD 支架成为促进细胞黏附和增殖的理想选择，从而加速全层皮肤缺损模型中的皮肤伤口愈合。这种酶交联策略为伤口愈合提供了有利的微环境，在皮肤再生工程中有很大的应用潜力。
