生长分化因子11对颞下颌关节骨关节炎小鼠髁突软骨细胞脂肪变性的影响

Effect of growth differentiation factor 11 on the steatosis of condylar chondrocytes in temporomandibular joint osteoarthritic mice.

作者信息

Wang Ruoxin, Liu Qian, He Feng, Zhang Mian, Wang Helin

机构信息

School of Stomatology, Zhengzhou University, Zhengzhou 450052, China.

State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shanxi International Joint Research Center for Oral Diseases, Dept. of Oral Anatomy and Physiology, The Stomatological Hospital of the Fourth Military Medical University, Xi'an 710032, China.

出版信息

Hua Xi Kou Qiang Yi Xue Za Zhi. 2022 Jan 25;40(1):14-21. doi: 10.7518/hxkq.2022.01.003.

DOI:10.7518/hxkq.2022.01.003

PMID:38596988

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8905263/

Abstract

OBJECTIVES

To observe the effect of growth differentiation factor 11 (GDF11) on the steatosis of condylar chondrocytes in temporomandibular joint (TMJ) osteoarthritic mice.

METHODS

Six-week-old female C57 mice were stimulated with unilateral anterior crossbite (UAC) and locally injected with GDF11. TMJ sections were stained with safranin O, and GDF11 and Adiponectin in the TMJ cartilage were detected by immunohistochemical staining. GDF11, Adiponectin, type Ⅱ collagen (Col-Ⅱ), Aggrecan, type Ⅰ collagen (Col-Ⅰ), fatty acid-binding protein 4 (FABP4), and Perilipin 1 mRNA were detected by real-time polymerase chain reaction (real-time PCR).

RESULTS

The thickness and safranin O-positive area of TMJ cartilage in the UAC groups, as well as the mRNA expression of Col-Ⅱ and aggrecan, significantly decreased. The ratio of Adiponectin-positive cells significantly increased. The mRNA expression levels of Col-Ⅰ, Adiponectin, FABP4, and Perilipin 1 significantly increased. The TMJ cartilage of the GDF11 injection groups significantly thickened, and the safranin O-positive area significantly increased. The mRNA expression of Col-Ⅱ and Aggrecan significantly increased. The ratio of Adiponectin-positive cells significantly decreased. The mRNA expression levels of Col-I, Adiponectin, FABP4, and Perilipin 1 significantly decreased.

CONCLUSIONS

Extensive steatosis of chondrocytes and decreased expression of GDF11 in the degenerated cartilage of TMJ osteoarthritis are induced by UAC stimulation. Supplementation of exogenous GDF11 can effectively inhibit the steatosis of chondrocytes.

摘要

目的

观察生长分化因子11（GDF11）对颞下颌关节（TMJ）骨关节炎小鼠髁突软骨细胞脂肪变性的影响。

方法

对6周龄雌性C57小鼠进行单侧前牙反合（UAC）刺激，并局部注射GDF11。对TMJ切片进行番红O染色，通过免疫组织化学染色检测TMJ软骨中的GDF11和脂联素。通过实时聚合酶链反应（实时PCR）检测GDF11、脂联素、Ⅱ型胶原（Col-Ⅱ）、聚集蛋白聚糖、Ⅰ型胶原（Col-Ⅰ）、脂肪酸结合蛋白4（FABP4）和 perilipin 1 mRNA。

结果

UAC组TMJ软骨的厚度和番红O阳性面积以及Col-Ⅱ和聚集蛋白聚糖的mRNA表达显著降低。脂联素阳性细胞比例显著增加。Col-Ⅰ、脂联素、FABP4和perilipin 1的mRNA表达水平显著增加。GDF11注射组的TMJ软骨显著增厚，番红O阳性面积显著增加。Col-Ⅱ和聚集蛋白聚糖的mRNA表达显著增加。脂联素阳性细胞比例显著降低。Col-I、脂联素、FABP4和perilipin 1的mRNA表达水平显著降低。