上调钙结合蛋白1，从而促进牙龈上皮细胞的增殖。
upregulates calbindin 1 and thus promotes the proliferation of gingival epithelial cells.
作者信息
Zhang Yuwei, He Yuxuan, Ding Yi, Liu Chengcheng
机构信息
State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Periodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2022 Jan 25;40(1):93-99. doi: 10.7518/hxkq.2022.01.014.
OBJECTIVES
This study aims to investigate the effect of calbindin 1 on the proliferation and apoptosis of gingival epithelial cells affected by .
METHODS
A model of infecting CA9-22 was established . At 24 h after infection, the expression of calbindin 1 (CALB1) was detected by real-time fluorescent quantitative polymerase chain reaction, Western blot, and immunofluorescence analyses. The expression of CALB1 was further inhibited by RNA interference. Cell proliferation was detected by BrdU analysis, and cell apoptosis was detected by caspase 3 activity. The expression of MDM2 and p53 was detected by Western blot analysis.
RESULTS
infection upregulated the expression of CALB1 in CA9-22 cells with multiplicity-dependent manner. CALB1 promoted the proliferation of CA9-22 cells, increased the expression of MDM2, and inhibited the expression of p53. Inhibiting CALB1 expression did not affect the inhibitory effect of infection on CA9-22 apoptosis.
CONCLUSIONS
infection can promote the proliferation of CA9-22 cells by increasing CALB1 expression. The related mechanism may be associated with MDM2-p53.
目的
本研究旨在探讨钙结合蛋白1对受[未提及具体因素]影响的牙龈上皮细胞增殖和凋亡的作用。
方法
建立[未提及具体病原体]感染CA9 - 22的模型。感染后24小时，通过实时荧光定量聚合酶链反应、蛋白质免疫印迹法和免疫荧光分析检测钙结合蛋白1（CALB1）的表达。通过RNA干扰进一步抑制CALB1的表达。采用BrdU分析检测细胞增殖，通过半胱天冬酶3活性检测细胞凋亡。通过蛋白质免疫印迹分析检测MDM2和p53的表达。
结果
[未提及具体病原体]感染以多倍体依赖方式上调CA9 - 22细胞中CALB1的表达。CALB1促进CA9 - 22细胞的增殖，增加MDM2的表达，并抑制p53的表达。抑制CALB1表达不影响[未提及具体病原体]感染对CA9 - 22细胞凋亡的抑制作用。
结论
[未提及具体病原体]感染可通过增加CALB1表达促进CA9 - 22细胞增殖。相关机制可能与MDM2 - p53有关。
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