Liu Biao, Fang Tian, Liang Lei, Hu Wen-Juan, Dong Min, Chen Li, Wu Wei, Yun Shi-Feng
Department of Laboratory Animals, General Hospital of Eastern Theater Command, Nanjing, Jiangsu 210002, China.
Zhonghua Nan Ke Xue. 2023 Mar;29(3):195-201.
To investigate the effects of different concentrations of Rauwolfia extract (RE) on the proliferation of prostate cells in the rat model of benign prostatic hyperplasia (BPH).
We randomly divided 48 male SD rats into six groups of an equal number, BPH model control, finasteride, low-concentration RE, medium-concentration RE, high-concentration RE and normal control, and established a BPH model in the former five groups by subcutaneous injection of testosterone propionate following castration. We treated the rats of the finasteride and RE groups intragastrically with finasteride solution at 5 mg/kg and RE at 5, 10 and 20 mg/kg respectively, and those of the model control and normal control groups with an equal dose of normal saline, all once a day for 28 consecutive days. Then, we killed all the animals, collected their prostate tissue, obtained the wet weight and volume of the prostate, the prostate index and the contents of serum T and dihydrotestosterone (DHT), observed the morphological changes of the prostate tissue by HE staining, counted the glands in the prostate tissue, measured the intraglandular area, and determined the expressions of PCNA and α-SMA by immunohistochemistry.
Compared with the rats of the normal control group, the BPH model controls showed significantly increased wet weight (［0.923 ± 0.15］ vs ［1.455 ± 0.52］ g, P < 0.05), volume (［1.035 ± 0.29］ vs ［1.687 ± 0.31］ ml, P < 0.05) and index of the prostate (［0.23 ± 0.04］% vs ［0.37 ± 0.15］%, P < 0.05), dilation, hyperemia and edema of the prostatic stroma and vessels, and proliferation rate of the prostatic cells, but remarkably decreased number of glands (［20.35 ± 3.83］ vs ［12.56 ± 2.58］, P < 0.05), epithelial thickness (［39.76 ± 5.20］ vs ［19.52 ± 1.52］ μm, P < 0.05) and intraglandular area (［12.3 ± 1.21］ vs ［5.96 ± 0.34］ ×103μm2, P < 0.05). In comparison with the BPH model controls, the animals treated with RE, especially in the high-concentration RE group, exhibited marked decreases in the weight (［1.455 ± 0.52］ vs ［0.862 ± 0.31］ g, P < 0.05), volume ( ［1.687 ± 0.31］ vs ［0.952 ± 0.28］ ml, P < 0.05) and index of the prostate (［0.37 ± 0.15］% vs ［0.22 ± 0.07］%, P < 0.05), dramatic improvement in the number of glands (［12.56 ± 2.58］ vs ［18.36 ± 1.25］, P < 0.05), epithelial thickness (［39.76 ± 5.20］ vs ［19.04 ± 3.89］ μm, P < 0.05) and intraglandular area (［5.96 ± 0.34］ vs ［10.25 ± 0.98］ ×103μm2, P<0.05］, P < 0.05), remarkable down-regulation of the expressions of PCNA and α-SMA, and significant reduction of the contents of serum T (［19.147 ± 3.214］ vs ［6.016 ± 1.978］ ng/ml, P < 0.05) and DHT (［9.052 ± 0.633］ vs ［2.532 ± 0.386］ ng/ml, P < 0.05).
Rauwolfia extract can inhibit the proliferation of prostate cells and relieve BPH symptoms in a concentration-dependent manner in rats with BPH.
探讨不同浓度的萝芙木提取物（RE）对良性前列腺增生（BPH）大鼠模型前列腺细胞增殖的影响。
将48只雄性SD大鼠随机分为6组，每组数量相等，分别为BPH模型对照组、非那雄胺组、低浓度RE组、中浓度RE组、高浓度RE组和正常对照组。对前5组大鼠进行去势后皮下注射丙酸睾酮建立BPH模型。分别对非那雄胺组和RE组大鼠灌胃给予5 mg/kg的非那雄胺溶液和5、10、20 mg/kg的RE，对模型对照组和正常对照组大鼠灌胃等剂量的生理盐水，均每日1次，连续28天。然后处死所有动物，采集前列腺组织，测量前列腺湿重、体积、前列腺指数以及血清T和双氢睾酮（DHT）含量，通过HE染色观察前列腺组织形态变化，计数前列腺组织中的腺体数量，测量腺体内面积，采用免疫组化法检测PCNA和α-SMA的表达。
与正常对照组大鼠相比，BPH模型对照组大鼠前列腺湿重（［0.923±0.15］vs［1.455±0.52］g，P<0.05）、体积（［1.035±0.29］vs［1.687±0.31］ml，P<0.05）和指数（［0.23±0.04］% vs［0.37±0.15］%，P<0.05）显著增加，前列腺间质和血管扩张、充血、水肿，前列腺细胞增殖率升高，但腺体数量（［20.35±3.83］vs［12.56±2.58］，P<0.05）、上皮厚度（［39.76±5.20］vs［19.52±1.52］μm，P<0.05）和腺体内面积（［12.3±1.21］vs［5.96±0.34］×103μm2，P<0.05）显著减少。与BPH模型对照组相比，RE处理组动物，尤其是高浓度RE组，前列腺重量（［1.455±0.52］vs［0.862±0.31］g，P<0.05）、体积（［1.687±0.31］vs［0.952±0.28］ml，P<0.05）和指数（［0.37±0.15］% vs［0.22±0.07］%，P<0.05）显著降低，腺体数量（［12.56±2.58］vs［18.36±1.25］，P<0.05）、上皮厚度（［39.76±5.20］vs［19.04±3.89］μm，P<0.05）和腺体内面积（［5.96±0.34］vs［10.25±0.98］×103μm2，P<0.05］，P<0.05）显著改善，PCNA和α-SMA表达明显下调，血清T（［19.147±3.214］vs［6.016±1.978］ng/ml，P<0.05）和DHT（［9.052±0.633］vs［2.532±0.386］ng/ml，P<0.05）含量显著降低。
萝芙木提取物可抑制BPH大鼠前列腺细胞增殖，并呈浓度依赖性缓解BPH症状。
