Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870, Frederiksberg, Denmark.
Department of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Copenhagen, Denmark.
Virology. 2024 Jul;595:110072. doi: 10.1016/j.virol.2024.110072. Epub 2024 Apr 2.
Porcine respiratory coronavirus (PRCV) was initially detected in Europe, and later in the United States of America (US), in the 1980s. In this study we obtained and compared PRCV sequences from Europe and the US, and investigated how these are related to transmissible gastroenteritis virus (TGEV) sequences. The whole genome sequences of Danish (1/90-DK), Italian (PRCV15087/12 III NPTV Parma), and Belgian PRCV (91V44) strains are presented. These sequences were aligned with nine other PRCV sequences from Europe and the US, and 43 TGEV sequences. Following alignment of the PRCV sequences, it was apparent that multiple amino acid variations in the structural proteins were distinct between the European and US strains. The alignments were used to build phylogenetic trees to infer the evolutionary relationships between the strains. In these trees, the European PRCV strains clustered as a separate group, whereas the US strains of PRCV all clustered with TGEVs.
猪呼吸冠状病毒（PRCV）最初于 20 世纪 80 年代在欧洲和后来的美利坚合众国（美国）被发现。在本研究中，我们获得并比较了来自欧洲和美国的 PRCV 序列，并研究了它们与传染性胃肠炎病毒（TGEV）序列的关系。呈现了丹麦（1/90-DK）、意大利（PRCV15087/12 III NPTV Parma）和比利时 PRCV（91V44）毒株的全基因组序列。将这些序列与来自欧洲和美国的其他 9 个 PRCV 序列和 43 个 TGEV 序列进行了比对。在对 PRCV 序列进行比对后，很明显，结构蛋白中的多个氨基酸变异在欧洲和美国毒株之间存在明显差异。使用这些比对构建了系统发育树，以推断这些毒株之间的进化关系。在这些树中，欧洲 PRCV 株系聚集为一个单独的组，而美国的 PRCV 株系均与 TGEVs 聚集在一起。
