Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
Endocrinology and Metabolism Research Center, Vali-Asr Hospital, Medical Sciences University of Tehran, Tehran, Islamic Republic of Iran.
Biomed Pharmacother. 2024 May;174:116525. doi: 10.1016/j.biopha.2024.116525. Epub 2024 Apr 9.
We previously showed the beneficial effect of L-Lysine (Lys), a chemical chaperone, on reducing diabetic complications in diabetic rats and type 2 diabetic patients. Herein, we evaluated the effect of Lys co-administration with Vitamin C and Zinc (Lys+VC+Zn), in diabetic rats.
The streptozotocin (50 mg/Kg) was injected into male adult Wistar rats to induce diabetes. Then, different groups of normal and diabetic rats were treated with Lys and Lys+VC+Zn for five months. So, there were 0.1 % Lys in the drinking water of both groups. The control groups received water alone. During the experiment, the body weight, and various parameters were determined in the blood, serum/plasma, and urine of the rats.
The determination of biochemical indexes confirmed diabetes induction and its complications in rats. Treatment with either Lys or Lys+VC+Zn resulted in reduced blood glucose and protein glycation (decreasing AGEs and HbA1c), increased insulin secretion, alleviated insulin resistance and HOMA-IR, improved lipid profile and HDL functionality (LCAT and PON1), enhanced antioxidant status (FRAP and AOPP), improved kidney function (decreased microalbuminuria, serum urea, and creatinine), and increased chaperone capacity (HSP70). Lys+VC+Zn showed better effects on these parameters than Lys alone.
The results of this study indicated that co-administration of Lys, a chemical chaperone, with two antioxidants (VC and Zn) potentiates its antidiabetic effects and prevent diabetic complications in rat model of diabetes.
我们之前已经证明了 L-赖氨酸（Lys）作为化学伴侣对减少糖尿病大鼠和 2 型糖尿病患者的糖尿病并发症的有益作用。在此，我们评估了 Lys 与维生素 C 和锌（Lys+VC+Zn）联合给药在糖尿病大鼠中的效果。
链脲佐菌素（50mg/Kg）注射到雄性成年 Wistar 大鼠中以诱导糖尿病。然后，正常和糖尿病大鼠的不同组分别用 Lys 和 Lys+VC+Zn 治疗五个月。因此，两组大鼠的饮用水中都含有 0.1%的 Lys。对照组只接受水。在实验过程中，测定大鼠的体重和血液、血清/血浆和尿液中的各种参数。
生化指标的测定证实了糖尿病的诱导及其在大鼠中的并发症。用 Lys 或 Lys+VC+Zn 治疗可降低血糖和蛋白质糖化（减少 AGEs 和 HbA1c），增加胰岛素分泌，减轻胰岛素抵抗和 HOMA-IR，改善血脂谱和 HDL 功能（LCAT 和 PON1），增强抗氧化状态（FRAP 和 AOPP），改善肾功能（减少微量白蛋白尿、血清尿素和肌酐），并增加伴侣蛋白容量（HSP70）。Lys+VC+Zn 对这些参数的作用优于 Lys 单独作用。
本研究结果表明，化学伴侣 Lys 与两种抗氧化剂（VC 和 Zn）联合给药可增强其抗糖尿病作用，并预防糖尿病大鼠模型中的糖尿病并发症。
