Chen Hsiu-Chao, Feng Wen-Wei, Audira Gilbert, Kurnia Kevin Adi, Hung San-Ho, Castillo Agnes L, Roldan Marri Jmelou M, Hsiao Chung-Der, Hung Chih-Hsin
Institute of Biotechnology and Chemical Engineering, I-Shou University, Da-Shu, Kaohsiung 84001, Taiwan; Department of Dermatology, E-Da Cancer Hospital, Kaohsiung 824005, Taiwan; Dr. Feng's Dermatology Clinic, Kaohsiung 811022, Taiwan.
Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 320314, Taiwan.
Neurotoxicology. 2024 May;102:68-80. doi: 10.1016/j.neuro.2024.04.003. Epub 2024 Apr 9.
Melamine-tainted products have been found in the market and raised issues about food safety. Recent studies done in rodents and humans demonstrated the toxicities of melamine, especially in causing kidney damage and bladder stone formation. However, very few studies assessed its behavior toxicity in organisms, including fish. Therefore, in this study, the researchers aim to determine whether sub-chronic exposure to melamine via oral and systematic administration could induce behavioral abnormality in zebrafish. After 14 days of systematic exposure to melamine at doses of 0.1 and 10 ppm levels, zebrafish were subjected to multiple behavioral assays. Results from both exposure routes showed that melamine indeed slightly increased fish locomotion and altered their exploratory behaviors in the novel tank assay. Furthermore, tightened shoaling formation was also displayed by the treated fish in the waterborne exposure group. However, melamine exposure did not cause any obvious alterations in fish behaviors during other behavioral tests. In addition, in comparison with previously published data on the behavior toxicities of several solvents in zebrafish, our phenomic analysis suggests the relatively low behavior toxicities of melamine via either systematic exposure or oral administration to zebrafish compared to those solvents. Nevertheless, our data indicate that the potential neurotoxicity of chronic low-dose melamine should not be ignored.
市场上已发现三聚氰胺污染的产品，并引发了食品安全问题。最近在啮齿动物和人类身上进行的研究证明了三聚氰胺的毒性，尤其是其会导致肾损伤和膀胱结石形成。然而，很少有研究评估其对包括鱼类在内的生物体的行为毒性。因此，在本研究中，研究人员旨在确定通过口服和全身给药亚慢性暴露于三聚氰胺是否会诱导斑马鱼出现行为异常。在以0.1和10 ppm的剂量对三聚氰胺进行14天的全身暴露后，对斑马鱼进行了多项行为测定。两种暴露途径的结果均表明，三聚氰胺确实会轻微增加鱼类的运动，并在新水箱试验中改变它们的探索行为。此外，在水暴露组中，经处理的鱼也表现出更紧密的聚群行为。然而，在其他行为测试中，三聚氰胺暴露并未导致鱼类行为出现任何明显变化。此外，与先前发表的关于几种溶剂对斑马鱼行为毒性的数据相比，我们的表型分析表明，与那些溶剂相比，三聚氰胺通过全身暴露或口服给药对斑马鱼的行为毒性相对较低。尽管如此，我们的数据表明，慢性低剂量三聚氰胺的潜在神经毒性不容忽视。
