Liu Lifeng, Ma Yanling, Zhao Heng, Guo Lin, Guo Yan, Liu Chun-Ming
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing, China.
Front Plant Sci. 2024 Mar 27;15:1384246. doi: 10.3389/fpls.2024.1384246. eCollection 2024.
Salt tolerance during seed germination is an important trait for direct seeding and low-cost rice production. Nevertheless, it is still not clear how seed germination under salt stress is regulated genetically.
In this study, genome-wide association studies (GWAS) were performed to decipher the genetic basis of seed germination under salt stress using 541 rice varieties collected worldwide.
Three quantitative trait loci (QTLs) were identified including on chromosome 3, on chromosome 5, and on chromosome 4. Assessment of candidate genes in these loci for their responses to salt stress identified a TATA modulatory factor () in . The expression of was up-regulated in both roots and shoots after exposure to salt stress, and knockout mutants exhibited delayed seed germination under salt stress. Haplotype analysis showed that rice varieties carrying displayed elevated salt tolerance during seed germination. These results provide important knowledge and resources to improve rice seed germination under salt stress in the future.
种子萌发期间的耐盐性是直播和低成本水稻生产的一个重要性状。然而，盐胁迫下种子萌发的遗传调控机制仍不清楚。
在本研究中，利用收集自世界各地的541个水稻品种进行全基因组关联研究（GWAS），以解析盐胁迫下种子萌发的遗传基础。
鉴定出三个数量性状位点（QTL），分别位于第3染色体上、第5染色体上和第4染色体上。对这些位点中的候选基因对盐胁迫的响应进行评估，在其中鉴定出一个TATA调控因子（）。盐胁迫处理后，该基因在根和地上部的表达均上调，且该基因的敲除突变体在盐胁迫下种子萌发延迟。单倍型分析表明，携带该基因的水稻品种在种子萌发期间表现出较高的耐盐性。这些结果为未来改善盐胁迫下水稻种子萌发提供了重要的知识和资源。
