Herz Z, Northey D, Lawyer M, First N L
Biol Reprod. 1985 Jun;32(5):1163-8. doi: 10.1095/biolreprod32.5.1163.
Thirty-two cows were inseminated near the uterotubal junction at various stages of the estrous cycle and slaughtered 16 h later to determine the effects of stage of the estrous cycle and tubal site of sperm recovery on the frequency of acrosome-reacted bull spermatozoa. Slaughter times were 46, 70, 144, or 168 h after each cow was injected with prostaglandin (PG) F 2 alpha or during the luteal phase of the estrous cycle. Sperm were recovered from the upper uterus and the isthmus and ampulla of the oviducts and stained for both viability and acrosome reaction. The highest frequency of acrosome-reacted sperm was found in the ampulla ipsilateral to a dominant follicle (largest follicle present) or recent ovulation and primarily at 70 h after PGF2 alpha (P less than 0.05). Also, fewer sperm were acrosome reacted prior to (46 h post-PGF2 alpha) and well after (168 h post-PGF2 alpha) estrus than during or immediately postestrus (70, 90, and 144 h post-PGF2 alpha; P less than 0.05). Except for two cows, one at 46 h and one at 70 h, all cows with more than 50% acrosome-reacted sperm in the ampulla had ovulated before slaughter. These data suggest that capacitated sperm become localized in the ampulla of the oviduct of the ovulatory side around the time of ovulation.
在发情周期的不同阶段，对32头奶牛在子宫输卵管交界处附近进行授精，16小时后屠宰，以确定发情周期阶段和精子回收的输卵管部位对顶体反应公牛精子频率的影响。屠宰时间为每头奶牛注射前列腺素（PG）F2α后46、70、144或168小时，或在发情周期的黄体期。从子宫上部以及输卵管的峡部和壶腹部回收精子，并对其活力和顶体反应进行染色。在与优势卵泡（存在的最大卵泡）同侧或近期排卵的壶腹部，发现顶体反应精子的频率最高，主要在PGF2α注射后70小时（P<0.05）。此外，与发情期或发情期刚结束时（PGF2α注射后70、90和144小时）相比，发情前（PGF2α注射后46小时）和发情后很久（PGF2α注射后168小时）顶体反应的精子较少（P<0.05）。除了两头奶牛，一头在46小时，一头在70小时，所有在壶腹部顶体反应精子超过50%的奶牛在屠宰前已经排卵。这些数据表明，获能精子在排卵时会在排卵侧输卵管的壶腹部定位。
