Department of Dermatology and Venereology Faculty Hospital and Medical Faculty of Charles University, Hradec Králové, 50002, Czech Republic.
Department of Medical Biophysics, Medical Faculty of Charles University, Hradec Králové, 50002, Czech Republic.
Int Immunopharmacol. 2024 May 10;132:112023. doi: 10.1016/j.intimp.2024.112023. Epub 2024 Apr 10.
Eosinophils and basophils are implicated in allergic reactions, and the molecule CD200 on B cells may have regulatory functions. Assessing the associations between the expression of CD200 on B lymphocytes and eosinophils and basophils helps unravel the complex immune interactions in atopic dermatitis, aiding in targeted therapeutic approaches.
The aim of our study is to evaluate the association between the count of eosinophils, basophils, CD16 eosinophils, CD203 basophils, the expression of activation marker CD200 on B cells and on their subsets in patients suffering from atopic dermatitis with and without dupilumab and in control group.
Altogether we examined 75 subjects: 45 patients suffering from atopic dermatitis -32 patients without dupilumab treatment, 13 patients with dupilumab treatment and 30 subjects as a control group. Immunophenotype was examined by flow cytometry in which monoclonal antibodies with fluorescent molecules were used. For statistical analysis we used non-parametric Kruskal-Wallis one-factor analysis of variance with post-hoc by Dunn's test with Bonferroni modification and the Spearman's rank correlation coefficient with calculation of R (%, percent of Variation Explained).
In patients with dupilumab therapy we confirmed the association between absolute eosinophils and expression of molecule CD200 on total B lymphocytes (in 23.9 %), non-switched (in 27.2 %), naive (in 25 %) and memory (in 20.3 %) B lymphocytes and between relative eosinophils and expression of CD200 on total B lymphocytes (in 22.8 % %), non-switched (in 29 %), naive (in 21.3 %) and memory (in 22.3 %) B lymphocytes. This association is low in AD patients without dupilumab and even non linear in control healthy subjects.
The higher association between eosinophils and expression of CD200 molecule on memory, naive and non switched B lymphocytes in AD patients under dupilumab therapy suggests that activation of B lymphocytes is caused by IL-4, whose production involves eosinophils and the CD200 molecule on B lymphocytes.
嗜酸性粒细胞和嗜碱性粒细胞参与过敏反应，B 细胞上的分子 CD200 可能具有调节功能。评估 B 淋巴细胞和嗜酸性粒细胞、嗜碱性粒细胞上 CD200 的表达之间的关联有助于阐明特应性皮炎中的复杂免疫相互作用，有助于针对性的治疗方法。
我们的研究旨在评估特应性皮炎患者和无度普利尤单抗治疗的特应性皮炎患者以及对照组患者中，嗜酸性粒细胞、嗜碱性粒细胞、CD16 嗜酸性粒细胞、CD203 嗜碱性粒细胞、B 细胞及其亚群上激活标志物 CD200 的计数之间的关联。
共检查了 75 例患者：45 例特应性皮炎患者-32 例未接受度普利尤单抗治疗，13 例接受度普利尤单抗治疗，30 例作为对照组。通过流式细胞术检查免疫表型，其中使用带有荧光分子的单克隆抗体。统计学分析采用非参数 Kruskal-Wallis 单因素方差分析，并用 Dunn 检验进行事后检验，Bonferroni 修正，以及 Spearman 秩相关系数，计算 R（%，解释变异的百分比）。
在接受度普利尤单抗治疗的患者中，我们证实了绝对嗜酸性粒细胞与总 B 淋巴细胞上分子 CD200 的表达之间的关联（在 23.9%）、非转换（在 27.2%）、幼稚（在 25%）和记忆（在 20.3%）B 淋巴细胞之间，以及相对嗜酸性粒细胞与总 B 淋巴细胞上 CD200 的表达之间的关联（在 22.8%）、非转换（在 29%）、幼稚（在 21.3%）和记忆（在 22.3%）B 淋巴细胞之间。在无度普利尤单抗治疗的 AD 患者中，这种关联较低，甚至在对照组健康受试者中呈非线性。
在接受度普利尤单抗治疗的 AD 患者中，嗜酸性粒细胞与记忆、幼稚和非转换 B 淋巴细胞上 CD200 分子之间的关联较高，提示 B 淋巴细胞的激活是由涉及嗜酸性粒细胞和 B 淋巴细胞上 CD200 分子的 IL-4 引起的。
