Agricultural Research Center, Central Laboratory of residue Analysis of Pesticides and Heavy Metals in Foods (QCAP), Ministry of Agriculture and Land Reclamation, Giza 12311, Egypt.
Agricultural Research Center, Central Laboratory of residue Analysis of Pesticides and Heavy Metals in Foods (QCAP), Ministry of Agriculture and Land Reclamation, Giza 12311, Egypt.
Food Chem. 2024 Aug 15;449:139223. doi: 10.1016/j.foodchem.2024.139223. Epub 2024 Apr 4.
Recently some major safety concerns have been raised on organic contaminants in widely consumed plants such as coffee. Hence, this study aimed to develop specifically optimized methods for determining organic contaminants, such as pesticides and polychlorinated biphenyls (PCBs), in coffee using GC-MS/MS and LC-MS/MS. QuEChERS method was used as a base extraction method, and 27 experiments were studied using design of experiments with categorical variables (extraction buffers, cleanup sorbents, and coffee roasting degree) to find the optimum method for each matrix type. The optimum method for green coffee was acetate buffer and chitosan for clean-up, while no-buffer extraction and the PSA + C18 method were ideal for light and dark-roasted coffee. The optimized methods were validated in accordance with SANTE/11312/2021. Furthermore, ten real samples (4 green, and 6 roasted) from the markets were analysed; ortho-phenylphenol was found in all the roasted coffee samples, and carbendazim was found in one green coffee sample.
最近，人们对广泛食用的咖啡等植物中的有机污染物提出了一些重大安全问题。因此，本研究旨在开发专门针对使用 GC-MS/MS 和 LC-MS/MS 测定咖啡中有机污染物（如农药和多氯联苯）的优化方法。QuEChERS 方法被用作基础提取方法，并使用带有分类变量（提取缓冲液、净化吸附剂和咖啡烘焙程度）的实验设计研究了 27 个实验，以找到每种基质类型的最佳方法。对于绿咖啡豆，最佳方法是使用醋酸盐缓冲液和壳聚糖进行净化，而无缓冲液提取和 PSA+C18 方法则适用于浅度和深度烘焙的咖啡。优化方法按照 SANTE/11312/2021 进行了验证。此外，还对来自市场的 10 个实际样品（4 个绿咖啡豆和 6 个烘焙咖啡豆）进行了分析；所有烘焙咖啡样品中均检出邻苯基苯酚，一个绿咖啡豆样品中检出多菌灵。
Molecules. 2025-4-26
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