Sun Yiping, Yang Xiaofang, Hu Jiayi, Ji Fuchun, Chi Huajian, Liu Ya, Hu Kan, Hao Fangfang, Wen Xiaodong
College of Pharmacy, Dali University, Dali, Yunnan 671000, China.
Key Laboratory of Green Chemistry & Technology of MOE, College of Chemistry, Sichuan University, Chengdu, Sichuan 610064, China.
Talanta. 2024 Jul 1;274:126036. doi: 10.1016/j.talanta.2024.126036. Epub 2024 Apr 3.
In this study, the one-step switchable hydrophilic solvent (SHS)-based effervescence tablet microextraction (ETME) was coupled with smartphone digital image colorimetry (SDIC) for the field detection of nickel ion (Ni) for the first time. Both extractant and CO were generated in situ when the novel SHS-based effervescence tablet was placed in the sample solution. The complexant 1-(2-pyridinylazo)-2-naphthaleno (PAN) dissolved from the effervescence tablet to form a stable complex with Ni, and the extractant was uniformly dispersed in the sample solution under the action of CO and fully in contact with Ni-PAN, which enabled efficient extraction of Ni. The color changes of the extraction phase were captured by smartphone, then a quantitative relationship between the concentrations of Ni and color intensity of images captured using a smartphone was established by customized applet WASDIC, which realized quantitative analysis of Ni in different samples. Under optimal conditions, the enhancement factor (EF) of the proposed method was 65.1, the limit of detection (LOD) and limit of quantification (LOQ) were 1.69 and 5.64 μg L, respectively. The developed method was successfully applied to the detection of trace Ni in the environmental samples and natural medicines. And the applicability of the method for use in field analysis was validated.
在本研究中，首次将基于一步切换亲水性溶剂（SHS）的泡腾片微萃取（ETME）与智能手机数字图像比色法（SDIC）联用，用于现场检测镍离子（Ni）。当新型基于SHS的泡腾片置于样品溶液中时，萃取剂和CO均原位生成。从泡腾片中溶解出来的络合剂1-（2-吡啶偶氮）-2-萘酚（PAN）与Ni形成稳定络合物，萃取剂在CO的作用下均匀分散在样品溶液中，并与Ni-PAN充分接触，从而实现对Ni的高效萃取。用智能手机捕捉萃取相的颜色变化，然后通过定制小程序WASDIC建立Ni浓度与智能手机拍摄图像颜色强度之间的定量关系，实现了不同样品中Ni的定量分析。在最佳条件下，该方法的增强因子（EF）为65.1，检测限（LOD）和定量限（LOQ）分别为1.69和5.64 μg L。所建立的方法成功应用于环境样品和天然药物中痕量Ni的检测，并验证了该方法用于现场分析的适用性。
