State Key Laboratory of Analytical Chemistry for Life Science, School of Life Sciences, Nanjing University, Nanjing, 210023, PR China.
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Science, Nanjing Forestry University, Nanjing, 210037, PR China.
Talanta. 2024 Jul 1;274:126071. doi: 10.1016/j.talanta.2024.126071. Epub 2024 Apr 7.
The construction of hybrid materials is significant for the exploration of functionalities in colorimetric biosensing due to its structural designability and synergy effects. In this work, a COF-on-MOF hybrid nanomaterial has been newly synthesized for colorimetric biosensing. Experimental results reveal that on-surface synthesis of COF on MOF brings nanoscale proximity between COF and MOF, which exhibits more than two folds of peroxidase-like activity as compared to single Fe-MOF. Therefore, by using the MCA@Fe-MOF nanomaterial with the assist of a specific acetyl-peptide, MCA@Fe-MOF can serve as an efficient signal reporter for colorimetric assay of histone deacetylase (HDAC), and the limit of detection (LOD) can be as low as 0.261 nM. Looking forward, the demand for diverse and promising COF-on-MOF nanomaterials with varied functionalities is anticipated, propelling further exploration of their role in colorimetric biosensing.
杂化材料的构建对于探索比色生物传感中的功能具有重要意义，因为其具有结构设计性和协同效应。在这项工作中，新合成了一种 COF-on-MOF 杂化纳米材料用于比色生物传感。实验结果表明，在 MOF 表面合成 COF 带来了 COF 和 MOF 之间的纳米级接近，其过氧化物酶样活性比单个 Fe-MOF 高出两倍以上。因此，通过使用 MCA@Fe-MOF 纳米材料和特定的乙酰肽辅助，MCA@Fe-MOF 可以用作组蛋白去乙酰化酶（HDAC）比色分析的有效信号报告器，检测限（LOD）可以低至 0.261 nM。展望未来，预计会有更多具有不同功能的多样化和有前途的 COF-on-MOF 纳米材料的需求，这将推动它们在比色生物传感中的作用的进一步探索。
