Tarumanagara Human Cell Technology Laboratory, Faculty of Medicine, Tarumanagara University, Jakarta 11440, Indonesia.
Department of Biochemistry and Molecular Biology, Faculty of Medicine, Tarumanagara University, Jakarta 11440, Indonesia.
Biomed Mater. 2024 Apr 25;19(3). doi: 10.1088/1748-605X/ad3da4.
The standard surgical procedure for abdominal hernia repair with conventional prosthetic mesh still results in a high recurrence rate. In the present study, we propose a fibroblast matrix implant (FMI), which is a three-dimensional (3D) poly-L-lactic acid scaffold coated with collagen (matrix) and seeded with fibroblasts, as an alternative mesh for hernia repair. The matrix was seeded with fibroblasts (cellularized) and treated with a conditioned medium (CM) of human Umbilical Cord Mesenchymal Stem Cells (hUC-MSC). Fibroblast proliferation and function were assessed and compared between treated with CM hUC-MSC and untreated group, 24 h after seeding onto the matrix (= 3). To study the matricesthe hernia was surgically created on male Sprague Dawley rats and repaired with four different grafts (= 3), including a commercial mesh (mesh group), a matrix without cells (cell-free group), a matrix seeded with fibroblasts (FMI group), and a matrix seeded with fibroblasts and cultured in medium treated with 1% CM hUC-MSC (FMI-CM group).examination showed that the fibroblasts' proliferation on the matrices (treated group) did not differ significantly compared to the untreated group. CM hUC-MSC was able to promote the collagen synthesis of the fibroblasts, resulting in a higher collagen concentration compared to the untreated group. Furthermore, thestudy showed that the matrices allowed fibroblast growth and supported cell functionality for at least 1 month after implantation. The highest number of fibroblasts was observed in the FMI group at the 14 d endpoint, but at the 28 d endpoint, the FMI-CM group had the highest. Collagen deposition area and neovascularization at the implantation site were observed in all groups without any significant difference between the groups. FMI combined with CM hUC-MSC may serve as a better option for hernia repair, providing additional reinforcement which in turn should reduce hernia recurrence.
传统假体网片修补腹外疝的标准手术仍会导致高复发率。本研究提出了一种成纤维细胞基质植入物（FMI），它是一种三维（3D）聚左旋乳酸支架，表面涂有胶原蛋白（基质）并种植成纤维细胞，作为疝修补的替代网片。该基质种植了成纤维细胞（细胞化），并用人脐带间充质干细胞（hUC-MSC）的条件培养基（CM）处理。在接种到基质上 24 小时后（= 3），评估并比较了用 CM hUC-MSC 处理和未处理组的成纤维细胞增殖和功能，即 24 小时后。为了研究基质，在雄性 Sprague Dawley 大鼠上进行了疝手术，并使用四种不同的移植物（= 3）进行修复，包括商业网片（网片组）、无细胞基质（无细胞组）、种植成纤维细胞的基质（FMI 组）和种植成纤维细胞并在经 1% CM hUC-MSC 处理的培养基中培养的基质（FMI-CM 组）。检查显示，与未处理组相比，基质（处理组）上成纤维细胞的增殖没有显著差异。CM hUC-MSC 能够促进成纤维细胞的胶原合成，导致胶原浓度高于未处理组。此外，研究表明，基质允许成纤维细胞生长并在植入后至少 1 个月支持细胞功能。在 14 天终点时，FMI 组观察到最多的成纤维细胞，但在 28 天终点时，FMI-CM 组观察到最多的成纤维细胞。在所有组中都观察到植入部位的胶原沉积面积和新生血管化，但各组之间没有显著差异。FMI 与 CM hUC-MSC 联合使用可能是疝修补的更好选择，提供额外的加固，从而应降低疝复发率。
