Key Laboratory of Applied Biology and Aquaculture of Fish in Northern Liaoning Province, Dalian Ocean University, Dalian 116023, China; College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
Key Laboratory of Applied Biology and Aquaculture of Fish in Northern Liaoning Province, Dalian Ocean University, Dalian 116023, China; College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
Sci Total Environ. 2024 Jun 1;927:172381. doi: 10.1016/j.scitotenv.2024.172381. Epub 2024 Apr 10.
In fisheries, hypoxia stress is one of the most common environmental stresses that often lead to the death of large numbers of fish and cause significant economic losses. The pituitary, an important endocrine gland, lies below the hypothalamus region of the brain. It plays a crucial part in controlling vital physiological functions in fish, such as growth, reproduction, and responses to stress. However, the detailed mechanisms of how hypoxia affects these physiological processes via the pituitary remain largely unknown.
Fat greenlings (Hexagrammous otakii) were exposed to different dissolved oxygen (DO = 7. 6 mg/L and DO = 2 mg/L) for 24 h. miRNA-mRNA association analysis of H. otakii pituitary after hypoxia stress. Detecting apoptosis in H. otakii pituitary using Tunel and qPCR. Subsequent detection of hormones in H. otakii liver, gonads and serum by ELISA.
In this study, hypoxia causes immune system disorders and inflammatory responses through the combined analysis of miRNAs and mRNAs. Subsequent verification indicated a significant accumulation of reactive oxygen species (ROS) subsequent to hypoxia treatment. The overproduction of ROS cause oxidative stress and apoptosis in the pituitary, ultimately causing pituitary damage and reduced growth hormone and luteinising hormone release.
According to the association study of miRNA-mRNA, apoptosis problems caused by hypoxia stress result in H. otakii pituitary damage. In the meantime, this work clarifies the possible impact of hypoxia-stress on the pituitary cells, as well as on the gonadal development and growth of H. otakii.
在渔业中，缺氧应激是最常见的环境应激之一，常导致大量鱼类死亡，造成重大经济损失。脑垂体作为一个重要的内分泌腺，位于下丘脑区域下方。它在控制鱼类的重要生理功能方面起着至关重要的作用，如生长、繁殖和对压力的反应。然而，缺氧如何通过脑垂体影响这些生理过程的详细机制在很大程度上仍然未知。
将绿鳍鱼（Hexagrammous otakii）暴露于不同的溶解氧（DO=7.6mg/L 和 DO=2mg/L）中 24 小时。进行缺氧应激后绿鳍鱼脑垂体的 miRNA-mRNA 关联分析。使用 Tunel 和 qPCR 检测绿鳍鱼脑垂体中的细胞凋亡。随后通过 ELISA 检测绿鳍鱼肝脏、性腺和血清中的激素。
在这项研究中，通过 miRNAs 和 mRNAs 的综合分析，缺氧导致免疫系统紊乱和炎症反应。随后的验证表明，缺氧处理后会产生大量的活性氧（ROS）。ROS 的过度产生导致脑垂体的氧化应激和细胞凋亡，最终导致脑垂体损伤和生长激素和促黄体生成素释放减少。
根据 miRNA-mRNA 的关联研究，缺氧应激引起的细胞凋亡问题导致绿鳍鱼脑垂体损伤。同时，这项工作阐明了缺氧应激对脑垂体细胞以及绿鳍鱼性腺发育和生长的可能影响。
