Perel E, Daniilescu D, Kharlip L, Blackstein M E, Killinger D W
Mol Cell Endocrinol. 1985 Jul;41(2-3):197-203. doi: 10.1016/0303-7207(85)90023-1.
The conversion of androstenedione (A) to estrogens, testosterone (T) and 5 alpha-reduced metabolites was studied in different phases of cell growth in 4 lines of cultured human breast carcinoma cells. Aromatase activity was 10-fold greater in MD and DM than in MCF7 cells and was undetectable in ZR75 cells. Estrogen formation in MD and DM lines increased during the phase of exponential growth and decreased to 20% of maximum during confluence. 5 alpha-Reductase activity was determined by the formation of 5 alpha-androstane-3,17-dione (5 alpha-A-dione) and androsterone (AND), and was 5-fold greater in ZR75 cells than MD cells and 2-fold greater than in MCF7 cells. This activity was relatively constant during exponential growth and decreased during confluence. T accumulation was inversely related to 5 alpha-reductase activity. The MCF7 and ZR75 cells which contain estrogen receptors had the highest levels of 5 alpha-reductase activity while the MD line which lacks estrogen receptors had the lowest 5 alpha-reductase activity. The assessment of aromatase and 5 alpha-reductase activity in addition to estrogen and progesterone receptors may be helpful in predicting hormone sensitivity in human breast tumours.
在4株培养的人乳腺癌细胞系的不同细胞生长阶段，研究了雄烯二酮（A）向雌激素、睾酮（T）和5α-还原代谢产物的转化。MD和DM细胞系中的芳香化酶活性比MCF7细胞高10倍，而在ZR75细胞中未检测到。MD和DM细胞系中的雌激素生成在指数生长期增加，汇合时降至最大值的20%。5α-还原酶活性通过5α-雄甾烷-3,17-二酮（5α-A-二酮）和雄酮（AND）的生成来测定，ZR75细胞中的该活性比MD细胞高5倍，比MCF7细胞高2倍。该活性在指数生长期相对恒定，汇合时降低。T的积累与5α-还原酶活性呈负相关。含有雌激素受体的MCF7和ZR75细胞具有最高水平的5α-还原酶活性，而缺乏雌激素受体的MD细胞系具有最低的5α-还原酶活性。除了雌激素和孕激素受体外，评估芳香化酶和5α-还原酶活性可能有助于预测人乳腺肿瘤的激素敏感性。
