Department of Human Biology, School of Nutrition and Translational Research in Metabolism (NUTRIM), Faculty of Health, Medicine and Life Sciences, Maastricht University Medical Centre+, Maastricht, The Netherlands.
Int J Sport Nutr Exerc Metab. 2024 Apr 10;34(4):189-198. doi: 10.1123/ijsnem.2023-0264. Print 2024 Jul 1.
Whey protein ingestion during recovery from exercise increases myofibrillar but not muscle connective protein synthesis rates. It has been speculated that whey protein does not provide sufficient glycine to maximize postexercise muscle connective protein synthesis rates. In the present study, we assessed the impact of coingesting different amounts of collagen with whey protein as a nutritional strategy to increase plasma glycine availability during recovery from exercise. In a randomized, double-blind, crossover design, 14 recreationally active men (age: 26 ± 5 years; body mass index: 23.8 ± 2.1 kg·m-2) ingested in total 30 g protein, provided as whey protein with 0 g (WHEY), 5 g (WC05); 10 g (WC10), and 15 g (WC15) of collagen protein immediately after a single bout of resistance exercise. Blood samples were collected frequently over 6 hr of postexercise recovery to assess postprandial plasma amino acid kinetics and availability. Protein ingestion strongly increased plasma amino acid concentrations (p < .001) with no differences in plasma total amino acid availability between treatments (p > .05). The postprandial rise in plasma leucine and essential amino acid availability was greater in WHEY compared with the WC10 and WC15 treatments (p < .05). Plasma glycine and nonessential amino acid concentrations declined following whey protein ingestion but increased following collagen coingestion (p < .05). Postprandial plasma glycine availability averaged -8.9 ± 5.8, 9.2 ± 3.7, 23.1 ± 6.5, and 39.8 ± 11.0 mmol·360 min/L in WHEY, WC05, WC10, and WC15, respectively (incremental area under curve values, p < .05). Coingestion of a small amount of collagen (5 g) with whey protein (25 g) is sufficient to prevent the decline in plasma glycine availability during recovery from lower body resistance-type exercise in recreationally active men.
乳清蛋白在运动后恢复期间的摄入可增加肌原纤维但不增加肌肉结缔组织蛋白合成率。有人推测，乳清蛋白不能提供足够的甘氨酸来最大限度地提高运动后肌肉结缔组织蛋白合成率。在本研究中，我们评估了在运动后恢复期间，同时摄入不同量的胶原蛋白作为一种增加血浆甘氨酸供应的营养策略，对乳清蛋白的影响。在一项随机、双盲、交叉设计中，14 名有规律锻炼的男性（年龄：26 ± 5 岁；体重指数：23.8 ± 2.1 kg·m-2）总共摄入 30 g 蛋白质，其中乳清蛋白提供 0 g（WHEY）、5 g（WC05）、10 g（WC10）和 15 g（WC15）的胶原蛋白。在单次抗阻运动后立即摄入。在运动后恢复的 6 小时内频繁采集血样，以评估餐后血浆氨基酸动力学和可用性。蛋白质摄入强烈增加了血浆氨基酸浓度（p <.001），但不同处理之间的血浆总氨基酸可用性没有差异（p >.05）。与 WC10 和 WC15 处理相比，WHEY 处理时血浆亮氨酸和必需氨基酸可用性的餐后增加更大（p <.05）。乳清蛋白摄入后，血浆甘氨酸和非必需氨基酸浓度下降，但胶原共摄入后增加（p <.05）。WHEY、WC05、WC10 和 WC15 组的餐后血浆甘氨酸可用性平均值分别为-8.9 ± 5.8、9.2 ± 3.7、23.1 ± 6.5 和 39.8 ± 11.0 mmol·360 min/L（增量曲线下面积值，p <.05）。在有规律锻炼的男性中，乳清蛋白（25 g）与少量胶原蛋白（5 g）共摄入足以防止下肢抗阻型运动后恢复期间血浆甘氨酸可用性下降。
