c-myc和c-myb这两种癌基因在淋巴系和髓系急性及慢性白血病中的表达水平研究。

Study of the levels of expression of two oncogenes, c-myc and c-myb, in acute and chronic leukemias of both lymphoid and myeloid lineage.

作者信息

Ferrari S, Torelli U, Selleri L, Donelli A, Venturelli D, Narni F, Moretti L, Torelli G

出版信息

Leuk Res. 1985;9(7):833-42. doi: 10.1016/0145-2126(85)90304-2.

Abstract

Total cellular RNA from a series of leukemic cell populations, both myeloid and lymphoid, as well as from normal circulating lymphocytes was analysed for the expression of two cellular oncogenes, c-myc and c-myb, by Northern blot hybridization assay. Expression of c-myc but not of c-myb was observed in unstimulated normal lymphocytes. Stimulation by PHA was shown to activate the expression of both genes. Remarkably different levels of expression of c-myc were observed in ALL, whereas in CLL the expression of c-myc was uniformly low or absent. Differential expression of c-myc was detected in AML as well as in CML, c-myb was differentially expressed in AML and ALL, and absent in CLL and CML. Other single cases of hemopoietic disorders were studied, but the expression of the two oncogenes was low or absent. Neither evident genome amplification nor genome rearrangements were detected in the cell DNAs digested with restriction endonucleases.

摘要

通过Northern印迹杂交分析，对一系列髓系和淋巴系白血病细胞群体以及正常循环淋巴细胞的总细胞RNA进行了两种细胞癌基因c-myc和c-myb表达的检测。在未受刺激的正常淋巴细胞中观察到c-myc的表达，但未观察到c-myb的表达。PHA刺激显示可激活这两个基因的表达。在急性淋巴细胞白血病（ALL）中观察到c-myc的表达水平显著不同，而在慢性淋巴细胞白血病（CLL）中，c-myc的表达普遍较低或缺失。在急性髓系白血病（AML）以及慢性髓系白血病（CML）中均检测到c-myc的差异表达，c-myb在AML和ALL中差异表达，而在CLL和CML中缺失。还研究了其他单例造血系统疾病，但这两种癌基因的表达均较低或缺失。在用限制性内切酶消化的细胞DNA中未检测到明显的基因组扩增或基因组重排。