Conti-Tronconi B M, Dunn S M, Barnard E A, Dolly J O, Lai F A, Ray N, Raftery M A
Proc Natl Acad Sci U S A. 1985 Aug;82(15):5208-12. doi: 10.1073/pnas.82.15.5208.
An alpha-bungarotoxin-binding protein was purified from chick optic lobe and brain by an improved method. Previous and present observations justify its designation as a brain nicotinic acetylcholine receptor (AcChoR). It contains subunits whose apparent molecular weights are somewhat larger than those of subunits of peripheral AcChoRs. The size of the optic lobe AcChoR complex is greater than that of the peripheral receptor when estimated from its sedimentation behavior. Brain AcChoR subunits can be specifically precipitated by a monoclonal antibody directed against chick muscle AcChoR. Amino-terminal amino acid sequence analysis was performed on AcChoR preparations and isolated subunits from the optic lobe and from the rest of the chick brain. The sequences obtained demonstrate that, at least for the lowest molecular weight component, the AcChoRs from different brain areas are identical and they are highly homologous to muscle AcChoR. It is concluded that the brain alpha-bungarotoxin-binding protein is indeed a nicotinic AcChoR and is encoded by a set of genes that is different from, but strongly related to, that for the muscle AcChoR.
采用改进方法从鸡视叶和脑中纯化出一种α-银环蛇毒素结合蛋白。先前及当前的观察结果证明将其命名为脑烟碱型乙酰胆碱受体（AcChoR）是合理的。它包含一些亚基，其表观分子量略大于外周AcChoRs亚基的分子量。根据其沉降行为估计，视叶AcChoR复合物的大小大于外周受体。脑AcChoR亚基可被针对鸡肌肉AcChoR的单克隆抗体特异性沉淀。对视叶和鸡脑其他部分的AcChoR制剂及分离出的亚基进行了氨基末端氨基酸序列分析。所得序列表明，至少对于最低分子量组分，来自不同脑区的AcChoRs是相同的，并且它们与肌肉AcChoR高度同源。得出的结论是，脑α-银环蛇毒素结合蛋白确实是一种烟碱型AcChoR，并且由一组与肌肉AcChoR不同但密切相关的基因编码。
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