Zentrum für Molekulare Biologie, Universität Heidelberg, Im Neuenheimer Feld 282, D-6900 Heidelberg, FRG.
EMBO J. 1986 Jul;5(7):1503-8. doi: 10.1002/j.1460-2075.1986.tb04389.x.
Acetylcholine is a major excitatory neurotransmitter in the central nervous system of insects. Using DNA probes of the Torpedo nicotinic acetylcholine receptor (AChR) we have isolated two overlapping cDNA clones encoding a putative neuronal AChR protein from the fruitfly, Drosophila melanogaster. The predicted mature protein consists of 497 amino acids, has a calculated mol. wt of 57 340 and shows extensive homology to known AChR subunits from different species along its entire amino acid sequence. Northern analysis revealed a hybridizing mRNA of 3.2 kb in late embryo and in pupae. Expression of the corresponding AChR gene thus characterizes periods of neuronal differentiation in Drosophila.
乙酰胆碱是昆虫中枢神经系统中的主要兴奋性神经递质。我们使用来自电鳐的尼古丁型乙酰胆碱受体 (AChR) 的 DNA 探针，从果蝇 Drosophila melanogaster 中分离出两个重叠的 cDNA 克隆，它们编码一种假定的神经元 AChR 蛋白。预测的成熟蛋白由 497 个氨基酸组成，计算出的分子量为 57340，并且在整个氨基酸序列中与来自不同物种的已知 AChR 亚基表现出广泛的同源性。Northern 分析显示在晚期胚胎和蛹中有 3.2kb 的杂交 mRNA。因此，相应的 AChR 基因的表达特征在于果蝇中的神经元分化时期。
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