Yavelow J, Collins M, Birk Y, Troll W, Kennedy A R
Proc Natl Acad Sci U S A. 1985 Aug;82(16):5395-9. doi: 10.1073/pnas.82.16.5395.
Experiments reported here indicate a crude soybean extract, if defatted with acetone, effectively blocks cell transformation in vitro. An active component of this crude extract is the Bowman-Birk trypsin and chymotrypsin inhibitor. The chymotrypsin-inhibitory region of the Bowman-Birk inhibitor is responsible for suppressing in vitro transformation. Another low molecular weight soybean trypsin inhibitor does not significantly suppress transformation. The Bowman-Birk inhibitor (i) has an irreversible effect on the transformation process, (ii) can suppress radiation-induced transformation even when added to cultures many days after the carcinogen exposure, and (iii) is effective in its ability to suppress transformation when present in the medium at a concentration as low as 0.125 nM.
此处报告的实验表明，粗制大豆提取物若用丙酮脱脂，可有效阻断体外细胞转化。这种粗提物的一种活性成分是鲍曼-伯克胰蛋白酶和糜蛋白酶抑制剂。鲍曼-伯克抑制剂的糜蛋白酶抑制区域负责抑制体外转化。另一种低分子量大豆胰蛋白酶抑制剂对转化的抑制作用不明显。鲍曼-伯克抑制剂（i）对转化过程具有不可逆作用，（ii）即使在致癌物暴露数天后添加到培养物中也能抑制辐射诱导的转化，（iii）当在培养基中的浓度低至0.125 nM时，其抑制转化的能力依然有效。
