Geoffroy J, Benzoni D, Sassard J
Prostaglandins Leukot Med. 1985 Jun;18(3):393-9. doi: 10.1016/0262-1746(85)90072-1.
In order to assess the role of the renally formed thromboxane A2 in humans, a new radioimmunoassay was developed for the determination of the urinary excretion of thromboxane B2, the stable metabolite of thromboxane A2. Urines were extracted with ethylacetate and thromboxane B2 was separated from the other primary prostaglandins by a reversed phase high performance liquid chromatography using water, acetonitrile and acetic acid (71:29:0.1) as mobile phase with an overall recovery of 44.1% +/- 1.3 (n = 42). The radioimmunoassay, performed on the fraction containing thromboxane B2 used a sensitive (less than 1pg) and highly specific antibody (final dilution: 1/460 800) that we have raised in the rabbit. The coefficient of variation was found lower than 5% in the usable part of the standard curve and the inter-assay reproducibility was at 7.3%. The 24-hour urinary excretion of thromboxane B2 measured in 26 healthy adults established at 109 +/- 12.9 ng/24h in men and at 99.5 +/- 9.2 ng/24 h in women. Therefore, it differed from that of the other prostaglandins by lower values devoid of sex-related differences.
为了评估肾生成的血栓素A2在人体中的作用，开发了一种新的放射免疫分析法，用于测定血栓素A2的稳定代谢产物血栓素B2的尿排泄量。尿液用乙酸乙酯萃取，血栓素B2通过反相高效液相色谱法与其他主要前列腺素分离，流动相为水、乙腈和乙酸（71:29:0.1），总回收率为44.1%±1.3（n = 42）。对含有血栓素B2的馏分进行的放射免疫分析使用了一种敏感（小于1皮克）且高度特异的抗体（最终稀释度：1/460 800），该抗体是我们在兔体内制备的。在标准曲线的可用部分，变异系数低于5%，批间重复性为7.3%。对26名健康成年人进行的测量显示，男性24小时尿中血栓素B2的排泄量为109±12.9纳克/24小时，女性为99.5±9.2纳克/24小时。因此，它与其他前列腺素不同，数值较低且无性别差异。
