The International Graduate Program of Veterinary Science and Technology (VST), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand; Center of Excellence in Fish Infectious Diseases (CE FID), Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand; Aquaculture and Aquatic Resources Management, Department of Food Agriculture and Bioresources, School of Environment, Resources and Development, Asian Institute of Technology, Pathum Thani, Thailand.
Aquaculture and Aquatic Resources Management, Department of Food Agriculture and Bioresources, School of Environment, Resources and Development, Asian Institute of Technology, Pathum Thani, Thailand.
Fish Shellfish Immunol. 2024 Jun;149:109557. doi: 10.1016/j.fsi.2024.109557. Epub 2024 Apr 10.
Immersion vaccination, albeit easier to administer than immunization by injection, sometimes has challenges with antigen uptake, resulting in sub-optimal protection. In this research, a new strategy to enhance antigen uptake of a heat-inactivated Vibrio harveyi vaccine in Asian seabass (Lates calcarifer) using oxygen nanobubble-enriched water (ONB) and positively charged chitosan (CS) was explored. Antigen uptake in fish gills was assessed, as was the antibody response and vaccine efficacy of four different combinations of vaccine with ONB and CS, and two control groups. Pre-mixing of ONB and CS before introducing the vaccine, referred to as (ONB + CS) + Vac, resulted in superior antigen uptake and anti-V. harveyi antibody (IgM) production in both serum and mucus compared to other formulas. The integration of an oral booster (4.22 × 10 CFU/g, at day 21-25) within a vaccine trial experiment set out to further evaluate how survival rates post exposure to V. harveyi might be improved. Antibody responses were measured over 42 days, and vaccine efficacy was assessed through an experimental challenge with V. harveyi. The expression of immune-related genes IL1β, TNFα, CD4, CD8, IgT and antibody levels were assessed at 1, 3, and 7-day(s) post challenge (dpc). The results revealed that antibody levels in the group (ONB + CS) + Vac were consistently higher than the other groups post immersion immunization and oral booster, along with elevated expression of immune-related genes after challenge with V. harveyi. Ultimately, this group demonstrated a significantly higher relative percent survival (RPS) of 63 % ± 10.5 %, showcasing the potential of the ONB-CS-Vac complex as a promising immersion vaccination strategy for enhancing antigen uptake, stimulating immunological responses, and improving survival of Asian seabass against vibriosis.
浸泡免疫虽然比注射免疫更容易实施，但有时在抗原摄取方面存在挑战，导致保护效果不理想。在这项研究中，探索了一种新的策略，即在使用富氧纳米气泡水（ONB）和带正电荷壳聚糖（CS）增强热灭活哈维氏弧菌疫苗在亚洲鲈鱼（Lates calcarifer）中的抗原摄取。评估了鱼鳃中的抗原摄取，以及四种不同组合的疫苗与 ONB 和 CS 以及两个对照组的抗体反应和疫苗效力。在引入疫苗之前先将 ONB 和 CS 预混合，称为（ONB+CS）+Vac，与其他配方相比，在血清和黏液中均能更好地摄取抗原并产生抗哈维氏弧菌抗体（IgM）。在疫苗试验中，通过口服强化（4.22×10 CFU/g，在第 21-25 天）进一步评估了提高鱼类暴露于哈维氏弧菌后存活率的可能性。在 42 天内测量抗体反应，并通过哈维氏弧菌的实验性攻毒评估疫苗效力。在攻毒后 1、3 和 7 天（dpc）评估免疫相关基因 IL1β、TNFα、CD4、CD8、IgT 和抗体水平的表达。结果表明，在浸泡免疫和口服强化后，（ONB+CS）+Vac 组的抗体水平始终高于其他组，并且在哈维氏弧菌攻毒后免疫相关基因的表达也有所升高。最终，该组的相对存活率（RPS）为 63%±10.5%，表明 ONB-CS-Vac 复合物作为一种有前途的浸泡免疫策略，具有增强抗原摄取、刺激免疫反应和提高亚洲鲈鱼对弧菌病的存活率的潜力。
Fish Shellfish Immunol. 2019-9-20
Fish Shellfish Immunol. 2007-8
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