Department of Aquaculture, College of Fisheries, Karnataka Veterinary Animal and Fisheries Sciences University, Mangalore, 575002, India.
Department of Aquaculture, College of Fisheries, Karnataka Veterinary Animal and Fisheries Sciences University, Mangalore, 575002, India; Present: Department of Aquatic Environment, College of Fisheries, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141001, India.
Fish Shellfish Immunol. 2019 Nov;94:746-751. doi: 10.1016/j.fsi.2019.09.053. Epub 2019 Sep 20.
The present study evaluated the biofilm (BF) of Vibrio anguillarum for oral vaccination of Asian seabass, Lates calcarifer. An 80-day experiment was carried out in circular fiber-reinforced plastic (FRP) tanks using free cell (FC) and BF of Vibrio anguillarum with triplicate in each. Heat-inactivated FC and BF cells at 10, 10 and 10 CFU/g fish/d were fed to fish for 20 days, agglutination antibody titer estimated at each 10 days interval up to 60-day post vaccination. As compared to FC and control there was a significant increase in agglutinating antibody titer in the biofilm vaccinated fishes. Among the 3 doses, BF at 10 cfu/g fish/d was considered the ideal dose for vaccination. Relative percentage survival (RPS) was higher in biofilm vaccinated fish (85.4%) compared to that with free cells (27.0%). The study demonstrated the better performance of V. anguillarum biofilm oral vaccine compared that with free cell vaccine in L. calcarifer. The study further supports better performance of biofilm vaccine model with one more bacterial pathogen in a high carnivore fish.
本研究评估了鳗弧菌的生物膜（BF）在卵形鲳鲹（Lates calcarifer）口服疫苗中的应用。在圆形纤维增强塑料（FRP）罐中进行了为期 80 天的实验，每个实验重复 3 次，使用游离细胞（FC）和鳗弧菌 BF。将 10、10 和 10 CFU/g 鱼/d 的热灭活 FC 和 BF 细胞投喂给鱼 20 天，在接种后 60 天内每隔 10 天评估一次凝集抗体效价。与 FC 和对照组相比，生物膜接种鱼的凝集抗体效价显著升高。在 3 个剂量中，以 10 cfu/g 鱼/d 的 BF 被认为是接种的理想剂量。生物膜接种鱼的相对百分比存活率（RPS）（85.4%）高于游离细胞（27.0%）。本研究表明，与游离细胞疫苗相比，鳗弧菌生物膜口服疫苗在卵形鲳鲹中的表现更好。该研究进一步支持了在高肉食性鱼类中使用另一种细菌病原体的生物膜疫苗模型具有更好的性能。
