Strukova S M, Petrova L N, Kireeva E G, Rtishcheva G V, Liubimov V S
Vopr Med Khim. 1985 May-Jun;31(3):99-103.
Effect of inactive precursor of alpha-thrombin--prethrombin (Pre-I) and prostaglandin (PGE2) on aggregation of washed rat platelets induced by alpha-thrombin was studied. Pre-I inhibited dose-dependently the alpha-thrombin-induced platelet aggregation. K1 values for Pre-I of high and low affinity binding sites were equal to 0.9.10(-8) M and 0.56.10(-6) M, respectively. PGE2 inhibited the alpha-thrombin-induced aggregation after simultaneous addition and within 5 and 15 min of incubation with PGE2. Preincubation of platelets with alpha-thrombin within 15 min prevented the PGE2-induced aggregation but preincubation of platelets with Pre-I within 15 min did not influence the platelet aggregation, induced by PGE2.
研究了凝血酶原（Pre-I）这一凝血酶无活性前体和前列腺素（PGE2）对α-凝血酶诱导的洗涤大鼠血小板聚集的影响。Pre-I剂量依赖性地抑制α-凝血酶诱导的血小板聚集。高亲和力和低亲和力结合位点的Pre-I的K1值分别等于0.9×10⁻⁸M和0.56×10⁻⁶M。同时加入PGE2以及在与PGE2孵育5分钟和15分钟内，PGE2均抑制α-凝血酶诱导的聚集。在15分钟内用α-凝血酶预孵育血小板可阻止PGE2诱导的聚集，但在15分钟内用Pre-I预孵育血小板并不影响PGE2诱导的血小板聚集。
