Marković Milena, Vidaković Vera, Popović Zorica
Department of Ecology, Institute for Biological Research "Siniša Stanković"-National Institute of Republic of Serbia, University of Belgrade, 11108 Belgrade, Serbia.
Plants (Basel). 2024 Mar 30;13(7):993. doi: 10.3390/plants13070993.
Landmark-based geometric morphometrics (GM) was used to examine, for the first time, spontaneous hybridization between (L.) Moench and Vít, Douda and Mandák, and to assess inter- and intrapopulation variability in leaf shape, leaf size and venation in natural populations in Serbia (Western Balkans). Two geographically distant (30 km) and two close (1.2 km) populations were selected to examine hybridization. The variability in leaf shapes was assessed by canonical variate analysis and linear discriminant analysis performed on the symmetric component of variation. Covariation between the symmetric component of shape variation and the number of pairs of secondary leaf veins was investigated with partial least squares analysis. Static allometry was examined for the first time in the genus Mill. A higher proportion of leaves was classified as in geographically close populations, which is in accordance with the hypothesis about spontaneous hybridization. No single leaf of was classified as , indicating that putative hybrids can only be found in grey alder populations. This study demonstrates that GM is a powerful tool for species delimitation and hybrid detection in the genus and it can be used for preliminary screening in hybrid zones.
基于地标点的几何形态测量学（GM）首次被用于研究欧洲赤杨（L.）Moench和意大利赤杨Vít、Douda及Mandák之间的自然杂交现象，并评估塞尔维亚（西巴尔干地区）自然种群中叶形、叶大小和叶脉的种群间及种群内变异性。选择了两个地理距离较远（30公里）和两个距离较近（1.2公里）的种群来研究杂交情况。通过对变异的对称成分进行典型变量分析和线性判别分析来评估叶形的变异性。用偏最小二乘法分析形状变异的对称成分与次生叶脉对数之间的协变关系。首次在桤木属Mill中研究了静态异速生长。在地理距离较近的种群中，较高比例的欧洲赤杨叶被归类为意大利赤杨，这与关于自然杂交的假设相符。没有一片意大利赤杨叶被归类为欧洲赤杨，这表明假定的杂种仅能在灰桤木种群中发现。本研究表明，GM是桤木属物种界定和杂种检测的有力工具，可用于杂交区域的初步筛选。
