Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
College of Life Science, Northeast Forestry University, Harbin 150040, China.
Int J Mol Sci. 2024 Mar 25;25(7):3651. doi: 10.3390/ijms25073651.
L. possesses high economic value, but its growth and yield are seriously affected by salt stress. Though melatonin (MT) has been widely reported to enhance tolerance towards abiotic stresses in plants, the regulatory role melatonin plays in resisting salt tolerance in grapevines has scarcely been studied. Here, we observed the phenotypes under the treatment of different melatonin concentrations, and then transcriptome and metabolome analyses were performed. A total of 457 metabolites were detected in CK- and MT-treated cell cultures at 1 WAT (week after treatment) and 4 WATs. Exogenous melatonin treatment significantly increased the endogenous melatonin content while down-regulating the flavonoid content. To be specific, the melatonin content was obviously up-regulated, while the contents of more than a dozen flavonoids were down-regulated. Auxin response genes and melatonin synthesis-related genes were regulated by the exogenous melatonin treatment. WGCNA (weighted gene coexpression network analysis) identified key salt-responsive genes; they were directly or indirectly involved in melatonin synthesis and auxin response. The synergistic effect of salt and melatonin treatment was investigated by transcriptome analysis, providing additional evidence for the stress-alleviating properties of melatonin through auxin-related pathways. The present study explored the impact of exogenous melatonin on grapevines' ability to adapt to salt stress and provided novel insights into enhancing their tolerance to salt stress.
褪黑素（MT）已被广泛报道可增强植物对非生物胁迫的耐受性，但它在葡萄抵抗盐胁迫中的调节作用却鲜有研究。在这里，我们观察了不同褪黑素浓度处理下的表型，然后进行了转录组和代谢组分析。在处理后 1 周（WAT）和 4 周（WAT）时，在 CK 和 MT 处理的细胞培养物中检测到了 457 种代谢物。外源性褪黑素处理显著增加了内源性褪黑素含量，同时降低了类黄酮含量。具体来说，褪黑素含量明显上调，而十几种类黄酮的含量下调。生长素反应基因和褪黑素合成相关基因受外源性褪黑素处理的调节。WGCNA（加权基因共表达网络分析）鉴定出关键的盐响应基因；它们直接或间接地参与了褪黑素的合成和生长素的反应。通过转录组分析研究了盐和褪黑素处理的协同作用，通过生长素相关途径为褪黑素的缓解应激特性提供了额外的证据。本研究探讨了外源性褪黑素对葡萄适应盐胁迫能力的影响，为增强其耐盐性提供了新的思路。
