婴儿配方食品中β-半乳糖寡糖（GOS）的测定：协作研究，最终行动 2021.01.

Determination of β-Galactooligosaccharides (GOS) in Infant Formula: Collaborative Study, Final Action 2021.01.

机构信息

Société des Produits Nestlé S.A., Nestlé Research, Route du Jorat 57, 1000 Lausanne 26, Switzerland.

出版信息

J AOAC Int. 2024 Jul 4;107(4):679-692. doi: 10.1093/jaoacint/qsae031.

DOI:10.1093/jaoacint/qsae031

PMID:38614132

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11223758/

Abstract

BACKGROUND

We previously published a method for the determination of β-galactooligosaccharides (GOS) in infant formula and adult nutritionals, which is currently First Action AOAC Method 2021.01. In this study, reproducibility data were collected to support the promotion of the method to Final Action.

METHODS

A collaborative study was organized in which 14 laboratories from eight different countries participated. Initially, laboratories were requested to analyze two practice samples and request guidance from the study director in case of issues. Successful laboratories proceeded to analyze seven samples (six infant formula and one adult nutritional) received as blind duplicates.

RESULTS

Thirteen laboratories reported acceptable results for practice sample 1. Practice sample 2 could only be delivered to eight of the laboratories due to restrictions at customs. The 13 laboratories successfully analyzing practice sample 1 were requested to continue with the analysis of the multilaboratory trial (MLT) samples. Laboratory 14 was unable to solve some technical difficulties, so their data could not be used. Out of the seven samples tested, results for six infant formulas met the requirements of the AOAC Standard Method Performance Requirements (SMPR®) 2014.003, with repeatability (RSDr) ranging from 1.4 to 4.7% and reproducibility (RSDR) ranging from 8.1 to 11.6%. The adult nutritional sample returned results outside the range of the SMPR, having an RSDr of 9.9%, higher than the SMPR target of ≤6%, and an RSDR of 12.1%, just above the SMPR target of ≤12%.

CONCLUSION

The method described is suitable for the determination of GOS in infant formula.

HIGHLIGHT

A method is described which is suitable for the determination of GOS in infant formula.

摘要

背景

我们之前发表了一种测定婴儿配方食品和成人营养食品中β-半乳糖寡糖（GOS）的方法，目前该方法是 AOAC 第一法 2021.01。本研究收集了再现性数据，以支持将该方法推广为最终方法。

方法

组织了一项合作研究，来自 8 个不同国家的 14 个实验室参与了该研究。最初，要求实验室分析两个练习样品，并在有问题时向研究主任寻求指导。成功的实验室继续分析作为盲样重复的七个样品（六个婴儿配方食品和一个成人营养食品）。

结果

有 13 个实验室报告了练习样品 1 的可接受结果。由于海关限制，只有 8 个实验室能够收到练习样品 2。成功分析练习样品 1 的 13 个实验室被要求继续分析多实验室试验（MLT）样品。第 14 个实验室无法解决一些技术难题，因此其数据无法使用。在测试的 7 个样品中，6 个婴儿配方食品的结果符合 AOAC 标准方法性能要求（SMPR®）2014.003 的要求，重复性（RSDr）范围为 1.4%至 4.7%，再现性（RSDR）范围为 8.1%至 11.6%。成人营养样品的结果超出了 SMPR 的范围，重复性（RSDr）为 9.9%，高于 SMPR 的目标值≤6%，再现性（RSDR）为 12.1%，略高于 SMPR 的目标值≤12%。