β-半乳糖寡糖（GOS）在婴儿配方食品和成人营养食品中的测定：单实验室验证，2021.01 第一行动。
Determination of β-Galactooligosaccharides (GOS) in Infant Formula and Adult Nutritionals: Single-Laboratory Validation, First Action 2021.01.
机构信息
Société des Produits Nestlé S.A., Nestlé Research, Vers-Chez-Les-Blanc, 1000 Lausanne, Switzerland.
出版信息
J AOAC Int. 2022 Feb 4;105(1):142-158. doi: 10.1093/jaoacint/qsab095.
BACKGROUND
β-Galactooligosaccharides (GOS) are typically used in infant formula and adult nutritionals as a source of nondigestible oligosaccharides, which may bring beneficial effects through modulation of the gut microbiota. However, suitable methods for the determination of GOS in products with a high background of lactose do not exist.
OBJECTIVE
The aim of this work was to develop a method suitable for the determination of GOS in infant formula and adult nutritionals and demonstrate suitability through single laboratory validation.
METHODS
Reducing oligosaccharides are labeled with 2-aminobenzamide (2AB), separated by hydrophilic interaction LC, and determined assuming that all oligosaccharides give an equimolar response in the detector. The same sample is analyzed a second time after treatment with β-galactosidase to remove GOS. The difference in the determined oligosaccharides between the two measurements will be the GOS content of the sample. The method was validated in a single laboratory on infant formula and adult nutritionals.
RESULTS
Recoveries were in the range 91.5-102%, relative standards of deviation (RSDr) were in the range 0.7-5.99%, and one sample had an RSDr of 8.30%. Except for the one sample with an RSDr of 8.30%, the performance is within the requirements outlined in the Standard Method Performance Requirements, which specifies recoveries in the range 90-110% and RSDr of below 6%.
CONCLUSIONS
The method is suitable for the determination of GOS in infant formula and adult nutritionals.
HIGHLIGHTS
A method has been developed which is suitable for the determination of GOS in products with a high background concentration of lactose (infant fromula and adult nutritionals). The method does not require access to the GOS ingredient used for the production of the finished product. It is also possible to separately quantify the amount of GOS containing three or more monomeric units in order to support dietary fibre analysis.
背景
β-半乳糖寡糖（GOS）通常被用作婴儿配方食品和成人营养食品中的不可消化寡糖的来源，通过调节肠道微生物群可能带来有益的效果。然而，对于乳糖含量高的产品中 GOS 的测定，目前尚无合适的方法。
目的
本工作旨在开发一种适用于婴儿配方食品和成人营养食品中 GOS 测定的方法，并通过单实验室验证证明其适用性。
方法
还原型寡糖用 2-氨基苯甲酰胺（2AB）标记，通过亲水相互作用 LC 分离，并假定所有寡糖在检测器中给出等摩尔响应来进行测定。用β-半乳糖苷酶处理样品后，再次分析同一样品以去除 GOS。两次测量中确定的寡糖之间的差异将是样品中的 GOS 含量。该方法在婴儿配方食品和成人营养食品的单实验室中进行了验证。
结果
回收率在 91.5-102%范围内，相对标准偏差（RSDr）在 0.7-5.99%范围内，一个样品的 RSDr 为 8.30%。除了一个 RSDr 为 8.30%的样品外，其性能均在标准方法性能要求中规定的范围内，该要求规定回收率在 90-110%之间，RSDr 低于 6%。
结论
该方法适用于婴儿配方食品和成人营养食品中 GOS 的测定。
重点
已经开发出一种适用于测定高乳糖背景浓度（婴儿配方食品和成人营养食品）产品中 GOS 的方法。该方法不需要获得用于生产成品的 GOS 成分。也可以单独定量含有三个或更多单体单元的 GOS 量，以支持膳食纤维分析。
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