Patil Medical College, Hospital and Research Centre, Patil Vidyapeeth, Pimpri, Pune, India.
J Nepal Health Res Counc. 2024 Mar 22;21(3):417-427. doi: 10.33314/jnhrc.v21i3.4538.
Tumour budding Tuberculosis is a new prognostic marker whose role in breast cancer is still under evaluation. Our aim was to study Tuberculosis in breast carcinoma and correlate it with other prognostic markers.
A descriptive cross-sectional study was conducted over 2 years on 75 invasive breast carcinoma specimens and biopsies. Hematoxylin and Eosin sections were examined for tumour grade, stage, molecular subtype, necrosis, lymphovascular invasion inflammation and counting of Tuberculosis. Lymph node metastasis was studied only in mastectomies. TB was defined as a cluster of 1-5 tumour cells and counted in 10 consecutive 400X fields. The cut-off for high grade TB was taken as ?10 per 10 HPFs. Immunohistochemical staining was done for molecular subtyping and differentiating Tuberculosis from mimickers. Statistical analysis was done using chi square test and Fischer's exact test.
Tuberculosis was present in 66/75 cases; 53% (n=35) were high grade. Among these, majority were of T2 (74%, n= 26), grade 2 (52%, n= 18), luminal A (34%, n= 12), had 3+ inflammation (46%, n= 16) and peripheral tumour buds (54%). Necrosis and lymphovascular invasion were absent in 77% and 71%, respectively. Lymph node metastasis was seen in 63% (n= 25/28) cases. Statistically significant association (p= 0.016) was observed between degree of inflammation and Tuberculosis grade. However, no significant association was observed between TB and other prognostic markers of breast carcinoma.
In our study, association of Tuberculosis with different prognostic markers was appreciated but was not statistically significant. However, it highlights need for standardization of Tuberculosis reporting.
肿瘤浸润性微转移是一种新的预后标志物，其在乳腺癌中的作用仍在评估中。我们的目的是研究乳腺癌中的微转移，并将其与其他预后标志物进行相关性分析。
在 2 年内对 75 例浸润性乳腺癌标本和活检进行了描述性横断面研究。苏木精-伊红染色切片用于评估肿瘤分级、分期、分子亚型、坏死、淋巴血管侵犯、炎症和微转移计数。仅在乳房切除术标本中研究淋巴结转移。微转移定义为 1-5 个肿瘤细胞簇，在 10 个连续的 400X 视野中进行计数。高分级微转移的截断值为每 10 高倍视野中 10 个。进行免疫组织化学染色以进行分子亚型分析，并区分微转移与模拟物。使用卡方检验和 Fisher 精确检验进行统计学分析。
在 75 例病例中，有 66 例存在微转移；53%（n=35）为高分级。其中，大部分为 T2（74%，n=26）、2 级（52%，n=18）、管腔 A（34%，n=12），有 3+炎症（46%，n=16）和外周肿瘤芽（54%）。无坏死和淋巴血管侵犯分别占 77%和 71%。淋巴结转移见于 63%（n=25/28）的病例。炎症程度与微转移分级之间存在显著相关性（p=0.016）。然而，微转移与乳腺癌的其他预后标志物之间未观察到显著相关性。
在我们的研究中，微转移与不同的预后标志物之间存在相关性，但无统计学意义。然而，这突出了需要对微转移报告进行标准化。
