Yaşar Arife, Ryu Hye-Jung, Esen Emine, Sarıoğlan İhsan, Deemer Dane, Çetin Bülent, Yoo Sang-Ho, Lindemann Stephen R, Lee Byung-Hoo, Tunçil Yunus E
Food Engineering Department, Engineering Faculty, Necmettin Erbakan University, Konya 42090, Turkiye.
Department of Food Science & Biotechnology, Gachon University, Seongnam 13120, Republic of Korea.
Carbohydr Polym. 2024 Jul 1;335:122087. doi: 10.1016/j.carbpol.2024.122087. Epub 2024 Mar 26.
The aim of this study was to evaluate the impacts of enzymatically synthesized α-glucans possessing α-1,4- and α-1,6-glucose linkages, and varying in branching ratio, on colonic microbiota composition and metabolic function. Four different α-glucans varying in branching ratio were synthesized by amylosucrase from Neisseria polysaccharea and glycogen branching enzyme from Rhodothermus obamensis. The branching ratios were found to range from 0 % to 2.8 % using GC/MS. In vitro fecal fermentation analyses (n = 8) revealed that the branching ratio dictates the short-chain fatty acid (SCFA) generation by fecal microbiota. Specifically, slightly branched (0.49 %) α-glucan resulted in generation of significantly (P < 0.05) higher amounts of propionate, compared to more-branched counterparts. In addition, the amount of butyrate generated from this α-glucan was statistically (P > 0.05) indistinguishable than those observed in resistant starches. 16S rRNA sequencing revealed that enzymatically synthesized α-glucans stimulated Lachnospiraceae and Ruminococcus related OTUs. Overall, the results demonstrated metabolic function of colonic microbiota can be manipulated by altering the branching ratio of enzymatically synthesized α-glucans, providing insights into specific structure-function relationships between dietary fibers and the colonic microbiome. Furthermore, the slightly branched α-glucans could be used as functional carbohydrates to stimulate the beneficial microbiota and SCFAs in the colon.
本研究的目的是评估具有α-1,4-和α-1,6-葡萄糖键且分支率不同的酶促合成α-葡聚糖对结肠微生物群组成和代谢功能的影响。由多糖奈瑟氏菌的淀粉蔗糖酶和奥氏嗜热放线菌的糖原分支酶合成了四种分支率不同的α-葡聚糖。使用气相色谱/质谱法发现分支率范围为0%至2.8%。体外粪便发酵分析（n = 8）表明，分支率决定了粪便微生物群产生短链脂肪酸（SCFA）的情况。具体而言，与分支较多的α-葡聚糖相比，轻度分支（0.49%）的α-葡聚糖产生的丙酸量显著更高（P < 0.05）。此外，这种α-葡聚糖产生的丁酸量与抗性淀粉中观察到的丁酸量在统计学上无显著差异（P > 0.05）。16S rRNA测序显示，酶促合成的α-葡聚糖刺激了毛螺菌科和瘤胃球菌相关的OTU。总体而言，结果表明可以通过改变酶促合成α-葡聚糖的分支率来调控结肠微生物群的代谢功能，这为膳食纤维与结肠微生物群之间特定的结构-功能关系提供了见解。此外，轻度分支的α-葡聚糖可用作功能性碳水化合物，以刺激结肠中的有益微生物群和SCFA。
