Center for Cleft Lip and Palate Treatment, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Center for Ear Reconstruction, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
J Dent Res. 2024 May;103(5):546-554. doi: 10.1177/00220345241232317. Epub 2024 Apr 15.
The intricate formation of the palate involves a series of complex events, yet its mechanistic basis remains uncertain. To explore major cell populations in the palate and their roles during development, we constructed a spatiotemporal transcription landscape of palatal cells. Palate samples from C57BL/6 J mice at embryonic days 12.5 (E12.5), 14.5 (E14.5), and 16.5 (E16.5) underwent single-cell RNA sequencing (scRNA-seq) to identify distinct cell subsets. In addition, spatial enhanced resolution omics-sequencing (stereo-seq) was used to characterize the spatial distribution of these subsets. Integrating scRNA-seq and stereo-seq with CellTrek annotated mesenchymal and epithelial cellular components of the palate during development. Furthermore, cellular communication networks between these cell subpopulations were analyzed to discover intercellular signaling during palate development. From the analysis of the middle palate, both mesenchymal and epithelial populations were spatially segregated into 3 domains. The middle palate mesenchymal subpopulations were associated with tooth formation, ossification, and tissue remodeling, with initial state cell populations located proximal to the dental lamina. The nasal epithelium of the palatal shelf exhibited richer humoral immune responses than the oral side. Specific enrichment of Tgfβ3 and Pthlh signals in the midline epithelial seam at E14.5 suggested a role in epithelial-mesenchymal transition. In summary, this study provides high-resolution transcriptomic information, contributing to a deeper mechanistic understanding of palate biology and pathophysiology.
palate 的复杂形成涉及一系列复杂事件，但它的机械基础仍不确定。为了探索 palate 中的主要细胞群体及其在发育过程中的作用，我们构建了 palate 细胞的时空转录图谱。从 C57BL/6 J 小鼠的胚胎第 12.5 天（E12.5）、第 14.5 天（E14.5）和第 16.5 天（E16.5）的 palate 样本中进行单细胞 RNA 测序（scRNA-seq），以鉴定不同的细胞亚群。此外，还使用空间增强分辨率组学测序（stereo-seq）来描述这些亚群的空间分布。整合 scRNA-seq 和 stereo-seq 与 CellTrek 注释的 palate 发育过程中的间充质和上皮细胞成分。此外，分析这些细胞亚群之间的细胞通讯网络，以发现 palate 发育过程中的细胞间信号。从中 palate 的分析中，间充质和上皮群体都被空间分隔成 3 个区域。中 palate 间充质亚群与牙齿形成、骨化和组织重塑有关，初始状态细胞群体位于牙板附近。palate 架的鼻腔上皮显示出比口腔侧更丰富的体液免疫反应。E14.5 时中线上皮缝中的特定 Tgfβ3 和 Pthlh 信号富集表明其在上皮-间充质转化中的作用。总之，这项研究提供了高分辨率的转录组信息，有助于更深入地了解 palate 生物学和病理生理学的机制。
