Elleuch Jihen, Drira Marwa, Ghribi Imtinen, Hadjkacem Farah, Pierre Guillaume, Khemakhem Hamadi, Michaud Philippe, Fendri Imen, Abdelkafi Slim
Laboratoire de Génie Enzymatique et Microbiologie, Equipe de Biotechnologie des Algues, Ecole Nationale d'Ingénieurs de Sfax, University of Sfax, Sfax, Tunisia.
Laboratory of Biotechnology and Plant Improvement, Center of Biotechnology of Sfax, Sfax, Tunisia.
Environ Technol. 2025 Jan;46(1):46-58. doi: 10.1080/09593330.2024.2338456. Epub 2024 Apr 15.
Microbial extracellular polymeric substances (EPS) have recently emerged as significant contributors in diverse biotechnological applications. Extracellular polymeric substances (EPS), produced by a strain, have been studied for potential applications in a specific heavy metal (lead (Pb II)) removal from wastewater. The optimisation of operational parameters, mainly pH, Pb and EPS concentrations, using the Box-Behnken design (BBD) was undertaken to enhance lead uptake. The higher Pb adsorption capacity reached 2211.029 mg/g. Hydroxyl, carbonyl, carboxyl, phosphoric, and sulfhydryl groups were identified quantitatively as potential sites for Pb adsorption. EPS exhibited a notable flocculation rate of 70.20% in kaolin clay at a concentration of 15 mg/L. They demonstrated an emulsifying activity greater than 88%, showcasing their versatile potential for both sedimentation processes and stabilising liquid-liquid systems. EPS could be excellent nonconventional renewable biopolymers for treating water and wastewater.
微生物胞外聚合物（EPS）最近已成为多种生物技术应用中的重要贡献者。由某菌株产生的胞外聚合物（EPS）已被研究用于从废水中去除特定重金属（铅（Pb II））的潜在应用。采用Box-Behnken设计（BBD）对主要操作参数（pH值、铅和EPS浓度）进行优化，以提高铅的吸附量。较高的铅吸附容量达到2211.029 mg/g。定量鉴定出羟基、羰基、羧基、磷酸基和巯基为铅吸附的潜在位点。EPS在浓度为15 mg/L的高岭土中表现出显著的絮凝率，为70.20%。它们表现出大于88%的乳化活性，展示了其在沉淀过程和稳定液-液体系方面的多功能潜力。EPS可能是用于处理水和废水的优秀非传统可再生生物聚合物。
