Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Institute of Entomology, Guizhou University, Guiyang, China.
PLoS One. 2024 Apr 16;19(4):e0298668. doi: 10.1371/journal.pone.0298668. eCollection 2024.
Limax maximus, or great gray slug, is a common agriculture pest. The pest infests crops during their growth phase, creating holes in vegetable leaves, particularly in seedlings and tender leaves. A study was conducted to assess the insecticidal activity of Ageratina adenophora extract against these slugs. Factors such as fecundity, growth, hatching rate, offspring survival rate, protective enzyme activity, and detoxifying enzyme activity were examined in slugs exposed to the extract's sublethal concentration (LC50) for two different durations (24 and 48 h). The phytochemical variability of the extracts was also studied. The LC50 value of the A. adenophora extract against L. maximus was 35.9 mg/mL. This extract significantly reduced the hatching rate of eggs and the survival rate of offspring hatched from exposed eggs compared with the control. The lowest rates were observed in those exposed for 48 h. The survival, growth, protective enzyme, and detoxification activity of newly hatched and 40-day-old slugs decreased. The A. adenophora extract contained tannins, flavonoids, and saponins, possibly contributing to their biological effects. These results suggest that the extract could be used as an alternative treatment for slug extermination, effectively controlling this species.
鳞灰蝶，又称大灰蝶，是一种常见的农业害虫。这种害虫在作物生长阶段会侵害庄稼，在蔬菜叶片上造成洞，尤其是在幼苗和嫩叶上。有一项研究评估了紫茎泽兰提取物对这些鳞灰蝶的杀虫活性。研究检查了暴露于提取物亚致死浓度（LC50）下 24 和 48 小时后，鳞灰蝶的繁殖力、生长、孵化率、后代存活率、保护酶活性和解毒酶活性等因素。还研究了提取物的植物化学成分变异性。A. adenophora 提取物对 L. maximus 的 LC50 值为 35.9 mg/mL。与对照组相比，该提取物显著降低了暴露组的卵孵化率和暴露卵孵化的后代存活率。暴露时间为 48 小时时，这两个指标的降低幅度最大。新孵化的和 40 天龄的鳞灰蝶的生存、生长、保护酶和解毒活性下降。紫茎泽兰提取物中含有单宁、类黄酮和皂苷，可能对其生物效应有贡献。这些结果表明，该提取物可用作消灭鳞灰蝶的替代治疗方法，有效控制该物种。
