Lin C S, Irwin R, Chirikjian J G
Nucleic Acids Res. 1979 Aug 10;6(11):3651-60. doi: 10.1093/nar/6.11.3651.
A single polypeptide of leucyl-tRNA synthetase (LRS) has been purified from budding bakers' yeast by a modification of the procedure published earlier. On denaturing polyacrylamide gel electrophoresis LRS was one band corresponding to molecular weight of 120,000 +/- 5,000 daltons. Variable amounts of LRS with a similar molecular weight but which dissociated into equal subunits of 58,000 were also isolated. The affinities (KM) for substrates for this form of the enzyme were similar to those previously reported for the dimeric form of the enzyme.
通过对先前发表的方法进行改进，从出芽的面包酵母中纯化出了一条亮氨酰 - tRNA合成酶（LRS）多肽。在变性聚丙烯酰胺凝胶电泳上，LRS呈现为一条带，对应分子量为120,000 ± 5,000道尔顿。还分离出了不同量的具有相似分子量但可解离为58,000相等亚基的LRS。这种形式的酶对底物的亲和力（KM）与先前报道的该酶二聚体形式的亲和力相似。
