Kagawa Tomohiro, Mineda Ayuka, Nakagawa Tomotaka, Shinohara Ayaka, Arakaki Ryosuke, Inui Hiroaki, Noguchi Hiroki, Yoshida Atsuko, Kinouchi Riyo, Yamamoto Yuri, Yoshida Kanako, Kaji Takashi, Nishimura Masato, Iwasa Takeshi
Department of Obstetrics and Gynecology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8503, Japan.
Exp Ther Med. 2024 Mar 26;27(5):231. doi: 10.3892/etm.2024.12520. eCollection 2024 May.
Secreted frizzled-related proteins (SFRPs) are involved in the development of various types of cancer and function by suppressing the Wnt signaling pathway. To elucidate the clinical implications of SFRPs in uterine sarcoma, SFRP expression levels and their effects on uterine leiomyosarcoma cells were examined. Immunostaining for SFRP4 was performed on uterine smooth muscle, uterine fibroid and uterine leiomyosarcoma tissues. Additionally, the effects of SFRP4 administration on cell viability, migration and adhesion were evaluated in uterine leiomyosarcoma SKN cells using the WST-1 assay (Roche Diagnostics) and the CytoSelect™ 24-well Cell Migration Assay Kit and the CytoSelect™ 48-well Cell Adhesion Assay Kit. The expression levels of SFRP4 in uterine leiomyosarcoma tissues were lower than those in normal smooth muscle and uterine fibroid tissues. In addition, SFRP4 suppressed the viability and migration, and increased the adhesion ability of uterine leiomyosarcoma cells compared with in the control group. In conclusion, SFRP4 may suppress the viability and migration, and enhance the adhesion of sarcoma cells. These results suggested that SFRP4 could be considered as a novel therapeutic target for uterine sarcoma.
分泌型卷曲相关蛋白（SFRPs）参与多种类型癌症的发生发展，并通过抑制Wnt信号通路发挥作用。为阐明SFRPs在子宫肉瘤中的临床意义，对SFRP表达水平及其对子宫平滑肌肉瘤细胞的影响进行了检测。对子宫平滑肌、子宫肌瘤和子宫平滑肌肉瘤组织进行了SFRP4免疫染色。此外，使用WST-1检测法（罗氏诊断公司）以及CytoSelect™ 24孔细胞迁移检测试剂盒和CytoSelect™ 48孔细胞黏附检测试剂盒，评估了SFRP4给药对子宫平滑肌肉瘤SKN细胞活力、迁移和黏附的影响。子宫平滑肌肉瘤组织中SFRP4的表达水平低于正常平滑肌和子宫肌瘤组织。此外，与对照组相比，SFRP4抑制了子宫平滑肌肉瘤细胞的活力和迁移，并增强了其黏附能力。总之，SFRP4可能抑制肉瘤细胞的活力和迁移，并增强其黏附能力。这些结果表明，SFRP4可被视为子宫肉瘤的一种新型治疗靶点。
