Reddy B S, Tanaka T, Simi B
J Natl Cancer Inst. 1985 Oct;75(4):791-8.
The effect of various levels of dietary corn oil or trans fat on azoxymethane (AOM; CAS: 25843-45-2)-induced carcinogenesis was investigated in female F344 rats fed the AIN-76 semipurified diets. Starting at 5 weeks of age, groups of rats were fed the low-fat diet containing 5% corn oil (designated as low-fat control diet). At 7 weeks of age, all animals except the vehicle-treated controls, were given sc injections of AOM (15 mg/kg body wt, once weekly) for 3 weeks. After 1 week, groups of animals were transferred to semipurified diets containing 13.6% corn oil and 23.5% corn oil or high-fat diets containing 5.9% corn oil plus 5.9% trans fat plus 11.8% Oleinate (low trans fat), 5.9% corn oil plus 11.8% trans fat plus 5.9% Oleinate (intermediate trans fat), and 5.9% corn oil plus 17.6% trans fat (high trans fat). Fecal bile acids were measured in vehicle-treated rats. All animals were necropsied 34 weeks after the last AOM injection. The animals fed the 23.5% corn oil diet had a higher incidence of colon tumors than did those in the groups fed the 5 and 13.6% corn oil diets. There was no difference in colon tumor incidence between the 5 and 13.6% corn oil diet groups. The animals fed the high-fat diets containing low trans fat, intermediate trans fat, and high trans fat developed significantly fewer liver and colon tumors and more small intestinal tumors than did the rats fed 23.5% corn oil diet. The excretion of fecal deoxycholic acid, lithocholic acid, and 12-ketolithocholic acid was higher in animals fed the 23.5% corn oil diet compared to the excretion in animals fed the other diets.
在喂食AIN - 76半纯化日粮的雌性F344大鼠中，研究了不同水平的膳食玉米油或反式脂肪对氧化偶氮甲烷（AOM；CAS：25843 - 45 - 2）诱导致癌作用的影响。从5周龄开始，将大鼠分组喂食含5%玉米油的低脂日粮（指定为低脂对照日粮）。7周龄时，除溶剂处理的对照组外，所有动物均皮下注射AOM（15mg/kg体重，每周一次），持续3周。1周后，将动物分组转移至含13.6%玉米油和23.5%玉米油的半纯化日粮，或含5.9%玉米油 + 5.9%反式脂肪 + 11.8%油酸盐（低反式脂肪）、5.9%玉米油 + 11.8%反式脂肪 + 5.9%油酸盐（中反式脂肪）和5.9%玉米油 + 17.6%反式脂肪（高反式脂肪）的高脂日粮。测定了溶剂处理大鼠的粪便胆汁酸。在最后一次注射AOM后34周对所有动物进行尸检。喂食23.5%玉米油日粮的动物结肠肿瘤发生率高于喂食5%和13.6%玉米油日粮组的动物。5%和13.6%玉米油日粮组之间的结肠肿瘤发生率没有差异。喂食含低反式脂肪、中反式脂肪和高反式脂肪高脂日粮的动物，与喂食23.5%玉米油日粮的大鼠相比，发生的肝脏和结肠肿瘤明显较少，小肠肿瘤较多。与喂食其他日粮的动物相比，喂食23.5%玉米油日粮的动物粪便脱氧胆酸、石胆酸和12 - 酮石胆酸的排泄量更高。
