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辅助病毒 RNA 的非模板功能创造了最佳的复制条件，以增强卫星 RNA 的增殖。

The non-template functions of helper virus RNAs create optimal replication conditions to enhance the proliferation of satellite RNAs.

机构信息

College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, China.

出版信息

PLoS Pathog. 2024 Apr 17;20(4):e1012174. doi: 10.1371/journal.ppat.1012174. eCollection 2024 Apr.

DOI:10.1371/journal.ppat.1012174
PMID:38630801
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11057728/
Abstract

As a type of parasitic agent, satellite RNAs (satRNAs) rely on cognate helper viruses to achieve their replication and transmission. During the infection of satRNAs, helper virus RNAs serve as templates for synthesizing viral proteins, including the replication proteins essential for satRNA replication. However, the role of non-template functions of helper virus RNAs in satRNA replication remains unexploited. Here we employed the well-studied model that is composed of cucumber mosaic virus (CMV) and its associated satRNA. In the experiments employing the CMV trans-replication system, we observed an unexpected phenomenon the replication proteins of the mild strain LS-CMV exhibited defective in supporting satRNA replication, unlike those of the severe strain Fny-CMV. Independent of translation products, all CMV genomic RNAs could enhance satRNA replication, when combined with the replication proteins of CMV. This enhancement is contingent upon the recruitment and complete replication of helper virus RNAs. Using the method developed for analyzing the satRNA recruitment, we observed a markedly distinct ability of the replication proteins from both CMV strains to recruit the positive-sense satRNA-harboring RNA3 mutant for replication. This is in agreement with the differential ability of both 1a proteins in binding satRNAs in plants. The discrepancies provide a convincing explanation for the variation of the replication proteins of both CMV strains in replicating satRNAs. Taken together, our work provides compelling evidence that the non-template functions of helper virus RNAs create an optimal replication environment to enhance satRNA proliferation.

摘要

作为一种寄生性的代理，卫星 RNA（satRNA）依赖于同源辅助病毒来实现其复制和传播。在 satRNA 的感染过程中，辅助病毒 RNA 作为合成病毒蛋白的模板，包括 satRNA 复制所必需的复制蛋白。然而，辅助病毒 RNA 的非模板功能在 satRNA 复制中的作用尚未得到充分利用。在这里，我们采用了黄瓜花叶病毒（CMV）及其相关 satRNA 组成的研究良好的模型。在采用 CMV 转录复制系统的实验中，我们观察到一个出乎意料的现象，即轻度株 LS-CMV 的复制蛋白在支持 satRNA 复制方面表现出缺陷，而不像严重株 Fny-CMV 那样。与翻译产物无关，所有 CMV 基因组 RNA 都可以增强 satRNA 的复制，当与 CMV 的复制蛋白结合时。这种增强取决于辅助病毒 RNA 的募集和完全复制。使用开发的分析 satRNA 募集的方法，我们观察到来自两种 CMV 株的复制蛋白招募携带有正链 satRNA 的 RNA3 突变体进行复制的能力明显不同。这与两种 1a 蛋白在植物中结合 satRNA 的不同能力一致。差异为两种 CMV 株的复制蛋白在复制 satRNA 方面的差异提供了令人信服的解释。总之，我们的工作提供了令人信服的证据，证明辅助病毒 RNA 的非模板功能创造了一个优化的复制环境，以增强 satRNA 的增殖。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/fb9bc47b0df0/ppat.1012174.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/f45f019dc15a/ppat.1012174.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/1e27212358ab/ppat.1012174.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/5edc0be4cee7/ppat.1012174.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/99c8c64116d4/ppat.1012174.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/b18a40497096/ppat.1012174.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/bc98639bdf6a/ppat.1012174.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/a1f185a9a590/ppat.1012174.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/3d732a351956/ppat.1012174.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/e09b18a6d704/ppat.1012174.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/fb9bc47b0df0/ppat.1012174.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/f45f019dc15a/ppat.1012174.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/1e27212358ab/ppat.1012174.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/5edc0be4cee7/ppat.1012174.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/99c8c64116d4/ppat.1012174.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/b18a40497096/ppat.1012174.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/bc98639bdf6a/ppat.1012174.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/a1f185a9a590/ppat.1012174.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/3d732a351956/ppat.1012174.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/e09b18a6d704/ppat.1012174.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cca6/11057728/fb9bc47b0df0/ppat.1012174.g010.jpg

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