Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Department of Chemistry, The University of Western Ontario, London, Ontario, Canada N6A 5B7, Canada.
ACS Biomater Sci Eng. 2024 May 13;10(5):2880-2893. doi: 10.1021/acsbiomaterials.3c01165. Epub 2024 Apr 17.
Cobalt-chromium-molybdenum (CoCrMo) alloys are common wear-exposed biomedical alloys and are manufactured in multiple ways, increasingly using additive manufacturing processes such as laser powder bed fusion (LPBF). Here, we investigate the effect of proteins and the manufacturing process (wrought vs LPBF) and building orientation (LPBF- and ) on the corrosion, metal release, tribocorrosion, and surface oxide composition by means of electrochemical, mechanical, microscopic, diffractive, and spectroscopic methods. The study was conducted at pH 7.3 in 5 g/L NaCl and 5 mM 2-(-morpholino) ethanesulfonic acid (MES) buffer, which was found to be necessary to avoid metal phosphate and metal-protein aggregate precipitation. The effect of 10 g/L bovine serum albumin (BSA) and 2.5 g/L fibrinogen (Fbn) was studied. BSA and Fbn strongly enhanced the release of Co, Cr, and Mo and slightly enhanced the corrosion (still in the passive domain) for all CoCrMo alloys and most for LPBF-, followed by LPBF- and the wrought CoCrMo. BSA and Fbn, most pronounced when combined, significantly decreased the coefficient of friction due to lubrication, the wear track width and severity of the wear mechanism, and the tribocorrosion for all alloys, with no clear effect of the manufacturing type. The wear track area was significantly more oxidized than the area outside of the wear track. In the reference solution without proteins, a strong Mo oxidation in the wear track surface oxide was indicative of a pH decrease and cell separation of the anodic and cathodic areas. This effect was absent in the presence of the proteins.
钴铬钼（CoCrMo）合金是常见的受磨损暴露的生物医学合金，有多种制造方法，越来越多地采用增材制造工艺，如激光粉末床熔合（LPBF）。在这里，我们研究了蛋白质、制造工艺（锻造与 LPBF）和构建方向（LPBF-和 ）对腐蚀、金属释放、摩擦腐蚀和表面氧化物组成的影响，采用电化学、机械、微观、衍射和光谱方法。该研究在 pH 7.3 的 5 g/L NaCl 和 5 mM 2-(-吗啉代)乙磺酸（MES）缓冲液中进行，发现这是避免金属磷酸盐和金属-蛋白质聚集体沉淀所必需的。研究了 10 g/L 牛血清白蛋白（BSA）和 2.5 g/L 纤维蛋白原（Fbn）的影响。BSA 和 Fbn 强烈促进了 Co、Cr 和 Mo 的释放，并略微增强了所有 CoCrMo 合金的腐蚀（仍处于钝化区），对 LPBF-的增强最大，其次是 LPBF-和锻造 CoCrMo。BSA 和 Fbn 结合时，由于润滑作用，显著降低了 CoCrMo 所有合金的摩擦系数、磨损轨迹宽度和磨损机制的严重程度，以及摩擦腐蚀，对制造类型没有明显影响。磨损轨迹区域的氧化程度明显高于磨损轨迹外部区域。在没有蛋白质的参考溶液中，磨损轨迹表面氧化物中 Mo 的强烈氧化表明 pH 值下降和阳极和阴极区域的细胞分离。在存在蛋白质的情况下，这种影响不存在。
