Icriverzi Madalina, Florian Paula Ecaterina, Bonciu Anca, Dumitrescu Luminita Nicoleta, Moldovan Antoniu, Pelinescu Diana, Ionescu Robertina, Avram Ionela, Munteanu Cristian V A, Sima Livia Elena, Dinca Valentina, Rusen Laurentiu, Roseanu Anca
Institute of Biochemistry of the Romanian Academy 060031 Bucharest Romania
National Institute for Lasers, Plasma, and Radiation Physics 409 Atomistilor Street 077125 Magurele Romania
Nanoscale Adv. 2024 Feb 27;6(8):2038-2058. doi: 10.1039/d3na00947e. eCollection 2024 Apr 16.
In this study, hybrid bio-nanoporous peptides loaded onto poly(-isopropylacrylamide--butylacrylate) (pNIPAM--BA) coatings were designed and obtained matrix-assisted pulsed laser evaporation (MAPLE) technique. The incorporation of cationic peptides magainin (MG) and melittin (Mel) and their combination was tailored to target synergistic anticancer and antibacterial activities with low toxicity on normal mammalian cells. Atomic force microscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy as well as contact angle and surface energy measurements revealed the successful and functional incorporation of both the peptides within porous polymeric nanolayers as well as surface modifications ( variation in the pore size diameter, surface roughness, and wettability) after Mel, MG or Mel-MG incorporation compared to pNIPAM--BA. testing revealed the impairment of biofilm formation on all the hybrid coatings while testing with , and . Moreover, MG was shown to modulate the effect of Mel in the combined Mel-MG extract formulation released pNIPAM-platforms, thus significantly reducing cancer cell proliferation through apoptosis/necrosis as revealed by flow cytometry analysis performed on HEK293T, A375, B16F1 and B16F10 cells. To the best of our knowledge, Mel-MG combination entrapped in the pNIPAM--BA copolymer has not yet been reported as a new promising candidate with anticancer and antibacterial properties for improved utility in the biomedical field. Mel-MG incorporation compared to pNIPAM--BA in testing revealed the impairment of biofilm formation in all the hybrid formulations.
在本研究中，利用基质辅助脉冲激光蒸发（MAPLE）技术设计并获得了负载于聚（N-异丙基丙烯酰胺-丙烯酸丁酯）（pNIPAM-BA）涂层上的杂化生物纳米多孔肽。阳离子肽马盖宁（MG）和蜂毒素（Mel）及其组合的掺入旨在实现协同抗癌和抗菌活性，同时对正常哺乳动物细胞具有低毒性。原子力显微镜、扫描电子显微镜、X射线光电子能谱、傅里叶变换红外光谱以及接触角和表面能测量结果表明，与pNIPAM-BA相比，在掺入Mel、MG或Mel-MG后，两种肽成功且功能性地掺入了多孔聚合物纳米层中，同时实现了表面改性（孔径直径、表面粗糙度和润湿性的变化）。测试表明，所有杂化涂层上的生物膜形成均受到损害，同时对 、 和 进行了测试。此外，在从pNIPAM平台释放的Mel-MG联合提取物制剂中，MG被证明可调节Mel的作用，因此，通过对HEK293T、A375、B16F1和B16F10细胞进行的流式细胞术分析表明，可通过凋亡/坏死显著降低癌细胞增殖。据我们所知，包裹在pNIPAM-BA共聚物中的Mel-MG组合尚未作为一种具有抗癌和抗菌特性的新的有前景的候选物被报道，以改善其在生物医学领域的应用。与pNIPAM-BA相比，在 测试中掺入Mel-MG表明所有杂化制剂中的生物膜形成均受到损害。
